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Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essential part of your protein production workflow when using a yeast expression system. 

Culture

Product No. Description
Y22001-1KT EnPresso® Y Defined
Y1375-250G YPD medium
Y1375-1KG YPD medium
Y1500-250G YPD Agar
Y1500-1KG YPD Agar
84605-500G SD Agar
Y1501-20G Yeast Synthetic Drop-out Media Supplements: without uracil
Y1751-20G Yeast Synthetic Drop-out Media Supplements: without histidine
Y1896-20G Yeast Synthetic Drop-out Media Supplements: without lysine
Y1376-20G Yeast Synthetic Drop-out Media Supplements: without leucine
Y1876-20G Yeast Synthetic Drop-out Media Supplements: without tryptophan
Y0750-20G Yeast Synthetic Drop-out Media Supplements: without leucine and tryptophan
Y1771-20G Yeast Synthetic Drop-out Media Supplements: without uracil, leucine and tryptophan
Y2146-20G Yeast Synthetic Drop-out Media Supplements: without histidine, leucine and tryptophan
Y2021-20G Yeast Synthetic Drop-out Media Supplements: without histidine, leucine, tryptophan, and adenine
Y2001-20G Yeast Synthetic Drop-out Media Supplements: without histidine, leucine, tryptophan, and uracil
Y0626-250G Yeast Nitrogen Base (woAA)
Y0626-1KG Yeast Nitrogen Base (woAA)
Y1250-250G Yeast Nitrogen Base (wAA)
T2559-250G Tryptone
T2559-1KG Tryptone
G5516-100ML Glycerol
G5516-500ML Glycerol
Y0875-250G Yeast Extract
Y0875-1KG Yeast Extract

 

Transformation

Product No. Description
YEAST1-1KT Yeast transformation kit
L4158-100G lithium acetate
L4158-250G lithium acetate
T9285-100ML TE
D8418-100ML DMSO
D8418-500ML DMSO
D8418-1L DMSO

 

β-galactosidase Assays

Product No. Description
B9146-10MG X-gal
B9146-100MG X-gal
N1127-500MG ONPG
N1127-5G ONPG
S7795-500G Na2CO3
S7795-1KG Na2CO3
S3264-500G Na2HPO4 7H2O
S3264-1KG Na2HPO4 7H2O
S3139-500G NaH2PO4 H2O
S3139-1KG NaH2PO4 H2O
P9541-500G KCl
P9541-1KG KCl
M3409-10X1ML MgSO4 7H2O
M3409-100ML MgSO4 7H2O
D4551-250ML DMF
D4551-500ML DMF

 

Cell Lysis (protein extraction)

Product No. Description
C4482 CelLytic™ Y Cell Lysis Reagent
CYP-1 CelLytic Y Plus Kit
P7626-1G PMSF
P7626-5G PMSF
P7626-25G PMSF
B5525-10G Bromophenol Blue
B5525-25G Bromophenol Blue
93362-500G Trizma® base
U5378-500G Urea
U5378-1KG Urea
L3771-100G SDS
L3771-500G SDS
E6760-100G EDTA
E6760-500G EDTA
P8215-1ML Protease Inhibitor Cocktail
P8215-5ML Protease Inhibitor Cocktail
A1542-500G ammonium acetate
A1542-2.5KG ammonium acetate
S3014-500G NaCl
S3014-1KG NaCl
S3014-5KG NaCl

 

Plasmid Preparation

Product No. Description
P4557-100ML Phenol solution
P4557-400ML Phenol solution
C0549-1PT Chloroforom:Isoamyl alcohol 24:1
C0549-1QT Chloroforom:Isoamyl alcohol 24:1
E7023-500ML Ethanol
E7023-6X500ML Ethanol