Restriction Enzyme Buffer Reference

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer for each enzyme (table 2).

Table 1
Activity (%) of each Sigma restriction enzyme in each Sigma buffer. The preferred buffer for each enzyme is highlighted in bold.

  Buffer
Restriction Enzyme SA (B7531) SB (B8781) SL (B3782) SM (B3158) SH (B3657)
Acc I (R6142) 100 0-10 10-25 1-10 0-10
Alu I (R6885) 100 50-75 25-50 25-50 0-10
Apa I (R4258) 100 10-25 50-75 50-75 0-10
Bam HI (R0260) 100 100 75-100 100 25-50
Bcl I (R8631) 100 100 25-50 100 100
Bgl II (R6377) 100 100 25-50 100 100
Bln I (R3131) 25-50 50-75 0-10 25-50 100
Bsm I (R3635) 0-10 50-75 0-10 25-50 100
Cfo I (R1761) 75-100 50-75 100 50-75 25-50
Cla I (R7763) 100 100 75-100 100 100
Dde I (R4256) 50-75 75-100 25-50 25-50 100
Dpn I (R8381) 100 75-100 50-75 75-100 75-100
Dra I (R4381) 100 75-100 100 100 50-75
Ecl XI (R3884) 25-50 100 25-50 25-50 50-75
Eco RI (R6265) 100 100 25-50 50-75 100
Eco RV (R2756) 25-50 100 0-10 25-50 50-75
Hae III (R5628) 50-75 50-75 75-100 100 25-50
Hind III (R1137) 50-75 100 25-50 100 50-75
Hpa I (R8507) 100 25-50 25-50 50-75 25-50
Kpn I (R1258) 75-100 10-25 100 25-50 0-10
Nco I (R8761) 50-75 50-75 50-75 50-75 100
Nde I (R5509) 25-50 100 10-25 50-75 100
Nhe I (R5634) 100 25-50 100 100 10-25
Not I (R8506) 10-25 50-75 0-10 25-50 100
Nsi I (R5884) 50-75 100 10-25 50-75 100
Pst I (R7023) 25-50 25-50 10-25 25-50 100
Pvu I (R1508) 50-75 75-100 25-50 50-75 100
Pvu II (R2631) 25-50 25-50 25-50 100 25-50
Rsa I (R4756) 100 50-75 100 50-75 0-10
Sac I (R5268) 100 0-10 100 50-75 0-10
Sal I (R0754) 0-10 25-50 0-10 10-25 100
Sau 3A (R0762) 100 25-50 25-50 75-100 0-10
Sca I (R5007) 0-10 100 0-10 75-100 100
Sfi I (R8256) 25-50 25-50 75-100 100 25-50
Sma I (R4503) 100 0-10 0-10 0-10 0-10
Spe I (R5257) 75-100 75-100 75-100 100 100
Sph I (R7135) 50-75 75-100 25-50 100 75-100
Ssp I (R6759) 75-100 75-100 10-25 75-100 100
Taq I (R9507) 50-75 100 25-50 50-75 50-75
Xba I (R7260) 100 75-100 75-100 75-100 100
Xho I (R6759) 25-50 75-100 10-25 25-50 100


Table 2

The complete formulation of each Sigma Restriction Enzyme Buffer. The formulation of the storage buffer for each Sigma restriction enzyme is given on the catalog page (see links in table 1).

  Final concentration (mM)
  SA (B7531) SB (B8781) SL (B3782) SM (B3158) SH (B3657)
Tris-acetate 33        
Tris-HCl   10 10 10 50
Mg-acetate 10        
MgCl2   5 10 10 10
K-acetate 66        
NaCl   100   50 100
Dithioerythritol (DTE)     1 1 1
Dithiothreitol (DTT) 0.5        
2-Mercaptoethanol (BME)   1      
pH at 37°C 7.9 8.0 7.5 7.5 7.5