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Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Universal ProbeLibrary System User Statements and Application

Related Downloads

The Universal ProbeLibrary - A Versatile Tool for Quantitative Expression Analysis in the Zebrafish (PDF)
The comparison of the Universal ProbeLibrary-based qPCR to a conventional SYBR Green-I-based qPCR assay using embryonic zebrafish cDNA.

Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology (PDF)
A real-time quantification method for accurate, sensitive, and economical detection of plant miRNAs based upon the stem-loop RT primer approach.

qPCR Validation of Candidates for Modulators of the MAPkinase Signaling Pathway (PDF)
Use of the dual-color assays substantially reduces the number of reactions that need to be performed in order to permit a qualitative and quantitative comparison of gene expression levels.

Rapid Development of Real-Time RT-PCR Assays Using Universal ProbeLibrary: Applications for Dissecting Signaling Pathways by RNA Interference (PDF)
We used signal transduction systems in Drosophila as a model and developed assays that measure the mRNA concentration of target genes using the Drosophila Universal ProbeLibrary.