Standard DNA Synthesis

DNA Oligo Specifications

Synthesis Scales 0.025, 0.05, 0.2, 1.0, 10, and 15µmole
mg - gram quantity manufacturing available upon request
Purification Desalt, Cartridge, HPLC, PAGE
See Oligonucleotide Purification Guidelines
Length 10 – 120 bases
Backbone Phosphodiester / Phosphorothioate
Format Dry or In Solution (normalized)
Modifications Over 200 available, including dyes (fluorescent and non-fluorescent)
Packaging 2 mL - 50 mL Tubes, Microtiter Plates
Quality Controls MALDI-TOF MS, Electrospray Ionization MS, OD by UV Spectroscopy
Technical Datasheet
& Labels
Includes yield, Tm (melting temperature), MW, and µg/OD
Duplicate set of labels with each order
Additional Services Concentration adjustment
Aliquoting into tubes or well plates
MSQC traces available upon request

DNA Oligos in Plates

Sigma can format your oligos to best suit your application as well as your automation and information requirements.

Custom specifications can include:

  • 96 or 384 well plates: Various plate types available
  • Concentration normalization options with fixed or variable volumes
  • Dried or in solution (water or TE)

In addition to the common formats listed above, other options are available including:

  • 1-D or 2-D Barcoding
  • Customer Specified Plate and Buffers
  • Daughter Plates (Copies)
  • Forward & Reverse Primers in a Single Well

Quality

Each shipment includes data sheets for import into customers’ information systems containing well location, sequence, oligo name, lot number, MW, µg/OD, length, Tm, concentration and volume (in solution).

Additional quality control measures are in place to verify accuracy for custom formats. These measures may include:

  • Plate orientation and well position verification by mass spectrometry
  • Mixed Primers, i.e. Forward and Reverse in a single well, verified by mass spectrometry
  • Verification for machine readable barcodes (1D and 2D)
  • Visual inspection of all master and daughter plates prior to shipment

Learn more about our quality control and quality assurance.

Concentration Verification and Plate Identity

Sigma employs a variety of liquid-handling automation systems to normalize the oligo concentration. A multi-step normalization process ensures the concentrations are within a narrow range. At the end of the process, a final absorbance reading (measured at 260nm) ensures that every oligo is at the desired concentration.

Shipping Schedule

Scale (µmole) Desalt Cartridge HPLC PAGE
0.025 24 hours NA NA NA
0.05 1-2 days 1-2 days 3-4 days 3-4 days
0.2 1-2 days 1-2 days 3-4 days 3-4 days
1.0 1-2 days 1-2 days 3-4 days 3-4 days
Plates (non-modified)* 3-5 days 3-5 days Inquire Inquire
Greater than 1.0 µmole 10 days or less 10 days or less 10 days or less 10 days or less
Large quantity Inquire Inquire Inquire Inquire
Post-synthesis modified 7-10 days 7-10 days 7-10 days 7-10 days
All other modifications 5-7 days or less 5-7 days or less 5-7 days or less 5-7 days or less

* Large projects will be placed on a delivery schedule based upon the customers project needs.

Minimum Yield Guarantees

Non-Modified Oligos (O.D./µg)*
Scale (µmole) Desalt Cartridge HPLC PAGE
0.025 3/90 NA NA NA
0.05 5/150 1/30 1/30 0.5/15
0.2 12/360 3/90 2.5/75 1/30
1.0 40/1,200 12/360 13/390 5/150
10 400/12,000 NA 130/3,900 NA
15 600/18,000 NA 190/5,700 NA

* Guarantee is for 20 mers or longer. Shorter oligos may have fewer ODs.