TruPAGE™ Precast Gels - Get a Better Grip

TruPAGE

SDS-PAGE protein gels are sometimes difficult to handle and break easily during experimental handling. This can result in researchers wasting valuable sample (and time) piecing together broken gels which is not efficient, reproducible, nor reliable.

TruPAGE SDS-PAGE precast gels are tear-resistant to reduce handling failures that commonly occur during gel electrophoresis and downstream processing. TruPAGE offers this enhanced durability AND continues to provide the protein resolution that you expect.

Tough, Tested, Tru

Tough, Tested, Tru

Features and Benefits

  • Durable – resists tearing to stand up to experimental handling
  • High-Performance – extended full length resolving gels to provide improved protein resolution for better blots
  • Excellent Compatibility – compatible with popular gel running equipment
  • Hassle-Free – no comb to pull, no tape to remove
  • Long Shelf Life – up to 24 months stability from the date of manufacture
  • Fast – run times as low as 30 minutes
  • Great Value – gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity

Innovative Stacking Technology

TruPAGE precast gels have proprietary stacking technology resulting in improved protein resolution and crisp bands. TruPAGE gels have large well capacity (20–35 μL).

(A)

Innovative Stacking Technology

Chicken Muscle Extract resolved with TruPAGE and stained with InstantBlue™. Lanes: 1) 10 μg, 2) 20 μg, 3) 30 μg, 4) 40 μg.

(B)

Innovative Stacking Technology

Anti-Flag Western Blot using TruPAGE to resolve samples. Lanes: 1) 1 μg Hela Lysate + 100 ng Carboxy-terminal FLAG-BAP (C-FLAG-BAP), 2) 0.5 μg Hela Lysate + 50 ng C-FLAG-BAP, 3) 0.25 μg Hela Lysate + 25 ng C-FLAG-BAP, 4) 100 ng C-FLAG-BAP, 5) 50 ng C-FLAG-BAP, 6) 25 ng C-FLAG-BAP.

Migration Table

Migration Table

Conversion Chart

Gel Form TruPAGE™ Equivalent to NuPAGE® Mini-PROTEAN TGX
10 × 10 cm 10% ---------> 10%
10 × 10 cm 12% ---------> 12%
10 × 10 cm 4–12% --------->
10 × 10 cm 4–20% ---------> 4–12%
10 × 8 cm 10% ---------> 10%
10 × 8 cm 12% ---------> 12%
10 × 8 cm 4–12% ---------> 4–15%
10 × 8 cm 4–20% ---------> 4–20%
Well Volume 12-well (35 μL)
17-well (20 μL)
---------> 12-well (20 μL)
17-well (15 μL)
12-well (20 μL)
17-well (15 μL)

*Use specifically formulated, non-proprietary TruPAGE buffers exclusively with TruPAGE gels to ensure optimal reproducibility and consistent excellent results. TruPAGE buffer recipes are supplied online.

 

 

Cat. No. Buffers and Reagents
PCG3001-500ML TruPAGE SDS Running Buffer, 20x, TEA-Tricine
PCG3003-500ML TruPAGE SDS Express Running Buffer, 20x, MOPS
PCG3005-1ML TruPAGE DTT Sample Reducer, 10x
PCG3007-10ML TruPAGE Running Antioxidant, 800x
PCG3009-10ML TruPAGE LDS Sample Buffer, 4x
PCG3011-500ML TruPAGE Transfer Buffer, 20x

 

Cat. No. Product Description
PCG2001 10EA TruPAGE Precast Gels, 10%, 10 × 10 cm, 12-well
PCG2002 10EA TruPAGE Precast Gels, 12%, 10 × 10 cm, 12-well
PCG2003 10EA TruPAGE Precast Gels, 4–12%, 10 × 10 cm, 12-well
PCG2004 10EA TruPAGE Precast Gels, 4–20%, 10 × 10 cm, 12-well
PCG2005 10EA TruPAGE Precast Gels, 10%, 10 × 10 cm, 17-well
PCG2006 10EA TruPAGE Precast Gels, 12%, 10 × 10 cm, 17-well
PCG2007 10EA TruPAGE Precast Gels, 4–12%, 10 × 10 cm, 17-well
PCG2008 10EA TruPAGE Precast Gels, 4–20%, 10 x 10 cm, 17-well
PCG2009 10EA TruPAGE Precast Gels, 10%, 10 × 8 cm, 12-well
PCG2010 10EA TruPAGE Precast Gels, 12%, 10 × 8 cm, 12-well
PCG2011 10EA TruPAGE Precast Gels, 4–12%, 10 × 8 cm, 12-well
PCG2012 10EA TruPAGE Precast Gels, 4–20%, 10 × 8 cm, 12-well
PCG2013 10EA TruPAGE Precast Gels, 10%, 10 × 8 cm, 17-well
PCG2014 10EA TruPAGE Precast Gels, 12%, 10 × 8 cm, 17-well
PCG2015 10EA TruPAGE Precast Gels, 4–12%, 10 × 8 cm, 17-well
PCG2016 10EA TruPAGE Precast Gels, 4–20%, 10 × 8 cm, 17-well

 

Cassette Size Gel Box Mfg. Compatible Boxes
10 × 8 cm Bio-Rad Mini-Protean® II
Bio-Rad Mini-Protean® Tetra
Hoefer Hoefer® Tall Mighty Small™ (SE280)
10 × 10 cm Life Technologies™ X-cell™ and Bolt®
Lonza PAGEr® Minigel Chamber

 

For a complete listing of gels and corresponding buffers, visit
sigma.com/trupage