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KitAlysis™ Benchtop Inertion Box

KitAlysis header

Materials Included

KitAlysis™ Benchtop Inertion Box with Lid

Additional Materials Needed

  1. Tygon tubing (I.D. x O.D. 14 in. x 3/8 in.)
  2. 3-way (Y) plastic or glass tubing connector

Description

The Aldrich® KitAlysis™ Benchtop Inertion Box provides an inert environment to run oxygen sensitive cross-coupling reactions easily and robustly in a laboratory fume hood.

General Set Up

  1. Place the Inertion Box as close to your inert gas source as possible while allowing you good access to the box for kit set up.
  2. Cut 3 pieces of tubing minimizing any excess where possible.
        a. 1 piece to be attached from your inert gas source to the three way valve.
        b. Two identical pieces to be attached first to  3-way valve and then to either side of the KitAlysis™ Benchtop
            Inertion Box
  3. Once tubing is in place, turn nitrogen flow on until you can hear a steady flow of nitrogen coming through the box. This is roughly 1-2 full revolutions of the nitrogen inlet knob on the typical fume hood. In general: you should be able to hear the flow of nitrogen over the normal noise of your operating hood while carrying on a conversation at an “inside voice” level.
  4. NEVER TURN ON THE HEAT under the Inertion Box. The Reaction Block must be removed for heating and should never be heated directly inside in the Inertion Box.
Inertion box view


 

For Best Results

  1. Place the KitAlysis™ Benchtop  Inertion Box in a reasonable spot as far back from the sash as possible while still allowing good access to the box for reaction set up. This helps to eliminate the increased air flow towards the front of the hood from disrupting the nitrogen flow coming into the Inertion Box.
  2. Lower the sash as much as possible to reduce extraneous air flow while still allowing easy access to the Inertion Box.
  3. Do not run hood on “emergency flow” while you are using your Inertion Box.
  4. Always place your substrate reaction vials (or empty dummy vials) in the center holes on either side of the reaction block (see diagram below). This helps to maintain an even and consistent flow.
  5. Purge the box with the reaction block and lid in place for 5 minutes before adding chemicals. Purge an additional 5 minutes after placement of chemicals into the Inertion Box and before making substrate solutions/dosing to reaction wells.
  6. Always keep the flow of nitrogen on at the same level during the entire set up. The term “purge” means to simply let the Inertion Box sit under the regular nitrogen flow without any movement within the box.

To be used with: KitAlysis24-Well Reaction Block and Screwdriver Set

For best result, the block and the lid should be placed in the Inertion Box prior to the first degassing. See picture below for suggested placement.

Inertion box corner view


 

Materials