Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Assurance® EIA TRANSIA® PLATE

Assurance EIA WELLSAssurance® EIA (Enzyme ImmunoAssay) and TRANSIA® PLATE incorporate proven antibody-antigen "sandwich" assay technology for the detection of food borne pathogens in both product and environmental samples. Highly specific antibodies coated to polystyrene microtiter plate wells capture and bind the target antigen, if present. A detection antibody linked to a conjugate enzyme is introduced to form an antibody/antigen/antibody sandwich. Finally, a substrate is added and converted by the conjugate enzyme to produce a color change indicating the presence of the target pathogen. The extensive AOAC validations and built-in positive and negative controls combine to provide users with the necessary confidence in the results. The efficient 96-well format and available automation make Assurance EIA / TRANSIA® Plate an ideal solution for high volume testing.

Accurate and Reliable

Proven Enzyme Immunoassay (EIA) technology utilizing complex, proprietary antibody formulations to overcome the challenges of differential bacterial detection.

High Throughput Efficiency

96-well microtiter plate format is ideal for medium to high volume testing laboratories seeking cost effective pathogen detection options.

Increase Productivity with Automation

Our immunoassays automation systems can further reduce labor costs by performing the necessary pipetting, washing, reading and interpretation of ELISA results. Up to 186 tests can be completed in just 2 hours.

Key Tests/Pathogens

  • Salmonella
  • Listeria spp.
  • Listeria monocytogenes
  • EHEC
  • Staphylococcal Enterotoxins

Validations
 

Salmonella Listeria spp. Listeria monocytogenes EHEC
AOAC OMA 992.11
AOAC OMA 999.08
AOAC PTM 010602
AFNOR TRA 02/08-03/01
AFNOR TRA 02/09-07/07
NordVal 001
AOAC OMA 996.14
AOAC PTM 060802
AFNOR TRA 02/06-11/95
NordVal 002
AFNOR TRA 02/11-03/08 AOAC OMA 996.10

Cost Effective Pathogen Testing for Food and Environmental Samples

BCS Gemini

  • Complete automation for TRANSIA® PLATE and Assurance® EIA ELISA methods
  • Cost effective and flexible
  • Intuitive, robust, high-throughput system