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A Selection of New Applications on the Supelco Low Bleed SLB-5ms

By: Katherine Stenerson, Jay Jones, and Michael D. Buchanan, Reporter US Edition 24.4

Katherine Stenerson, Jay Jones, and Michael D. Buchanan kstenerson@sial.com

Introduction

In past Reporter articles, we have highlighted the many features and benefits of the new SLB-5ms column, especially for US EPA Method 8270. In addition to Method 8270, we have also investigated the use of this column for analyses in other applications. In this article, we will highlight several of these applications.

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Agricultural Chemicals on Golf Courses

The run-off from golf courses treated with a variety of pesticides represents a potential environmental problem. Specifically, in Japan, the increased use of these compounds on golf courses has raised concerns. Figure 1 shows the separation of 29 “golf course pesticides” (compounds classified as pesticides, insecticides, herbicides, and fungicides) that were included in a list published by the Japanese Ministry of the Environment in 2003, and were found in a large number of run-off samples taken from golf courses around the country (1).

Figure 1.Agricultural Chemicals on Golf Courses

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Drugs of Abuse

A challenge for forensic and clinical chemists is the need to rapidly screen for a wide range of possible drugs. As such, accuracy and sensitivity are critical. Figure 2 shows the separation of 16 common drugs of abuse on an SLB-5ms column. The inertness of the SLB- 5ms resulted in symmetrical peak shapes for these compounds. In addition, the low bleed of the SLB-5ms will allow for greater sensitivity and better quality mass spectral confirmation at trace levels.

Figure 2.Drugs of Abuse

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Agricultural Pesticides in Wine

Figure 3 demonstrates the usefulness of Solid Phase Microextraction (SPME) in the low-level extraction of agricultural pesticides from wine, and the use of the SLB- 5ms in the subsequent analysis. The pesticides chosen for the analysis represent a group of insecticides and fungicides that could be found in wines due to their use in commercial vineyards (2). These compounds contain a variety of polar functional groups, and the polyacrylate fiber provided the selectivity necessary for extraction from a wine matrix. The inertness and low bleed of the SLB-5ms enabled subsequent low-level analysis of these compounds by GC-MS.

Figure 3.Agricultural Pesticides in Wine

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Trichloroanisole in Wine

The ability of headspace SPME, in combination with analysis on the SLB-5ms, to detect a low level of 2,4,6- trichloroanisole (TCA) in wine is shown in Figure 4. TCA is often the source of the musty smell in wine resulting from tainted corks. Red wine obtained in a bottle fitted with a metal screw cap was spiked with TCA and the headspace was extracted using SPME. Analysis was done by GC-MS/SIM on the SLB-5ms. The compound was detected in a spiked wine sample at 5 parts-per-trillion (ppt), which is well below the 40 ppt sensory threshold level in red wines (3).

Figure 4.Trichloroanisole in Wine

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Conclusion

The SLB-5ms column has been designed to provide the user with the low bleed and inertness to meet the demands of today’s sensitive GC-MS and GC applications. Additional applications using the SLB-5ms can be viewed or downloaded at sigma-aldrich.com/slb. If you have an idea for an application that you would like to see on the SLB-5ms, or any Supelco column, please feel free to drop us an e-mail at the address listed at the beginning of this article.

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References

  1. News Release, FY 2003 Survey Results of Water Pollution by Agricultural Chemicals Used at Golf Courses, Japanese Ministry of the Environment, 11/12/2004, www.env. go.jp/en/press/2004/1112a.
  2. Soleas, G.J.; Yan, J.; Hom, K.; Goldberg, D.M., Multiresidue Analysis of Seventeen Pesticides in Wine by Gas Chromatography with Mass-selective Detection. J. Chromatogr. A, 2000, 882: 205-212.
  3. Riu, M.; Mestres, M.; Busto, O.; Guasch, J., Determination of 2,4,6-Trichloroanisole in Wines by Headspace Solid-phase Microextraction and Gas Chromatography-electron –capture detection. J. Chromatogr. A, 2002, 977: 1-8.

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