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DNAstable® Blood Protocol for preservation of DNA in whole blood at room temperature (tubes and 48 well plates)

Product Description

DNAstable Blood matrix is a technology for safe, stable, and convenient storage of whole blood and buffy coat at room temperature (15–25°C). This medium is completely dissolvable and ensures total sample recovery.

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Storage/Stability

DNAstable blood Tubes & Plates should be stored dry in their original unopened packaging before use.

Procedure

Sample Drying and Storage

  • Open tube or remove cover from 48-well plate.
  • Add 50-200 μl of whole blood into the center of the vessel containing DNAstable Blood matrix.
  • Mix the sample by placing the DNAstable blood 48-well plate or the tubes (placed in the provided carrier plate) on a Microtiter plate shaker, and shake for 15 min at 600-800 rpm.
  • Dry the uncovered sample completely under a laminar flow at room temperature (15–25°C); or by placing in a ventilated incubator; or oven set to 40°C. Drying times are dependent on the blood volume and the drying conditions.
  • Cap tube or cover plate with an AirPore Tape sheet after drying.
  • Store at room temperature (15-25°C) and protect from moisture by either:
         1) Storing in a dry storage cabinet or
         2) Heat seal the moisture barrier bag, enclosing the dried sample and desiccant packet.

Note: Drying times may vary depending on the humidity level in the laboratory.

Sample Recovery: Just Add Water

  • Add 300 μl of DNAse free H2O to the tube or well containing stored sample.
  • Place the plate or the tubes (placed in the tube carrier plate) on a thermal shaker, and incubate at room temperature with continuous shaking at 1000-1200 rpm until the blood is rehydrated. Rehydration time is dependent on the original blood volume and the storage time, and may vary from 1 to 2 hours until the sample returns to liquid form. To avoid cross contamination of samples, do not shake at higher speeds
  • Transfer the rehydrated blood mixtue to a clean microfuge tube and follow the kit protocol for manual or automated purification of DNA from blood.

Purified DNA samples can be used directly in downstream applications such as:

  • PCR
  • qPCR (see handbook for details on dilution factors)
  • Sequencing
  • STR Analysis
  • Whole Genome Amplification
  • Restriction Analysis

Materials

     
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