Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

DNAstable® Plus Protocol

Product No. 53091-016, 52091-026

Product Description

DNAstable Plus is a unique storage medium that preserves purified genomic DNA, at room temperature, in liquid format for up to one year, and as a drydown for longer storage periods. Dried DNAstable Plus allows for long-term stabilization of DNA samples with easy sample recovery by simply adding water.

Stabilize and Store Purified DNA at Room Temperature

DNAstable Plus enables you to preserve, store and ship your purified DNA samples at room temperature in liquid and dry state. It offers workflow flexibility with thermostability at even extreme temperatures and has the ability to handle high concentrations of DNA.  Recovery from dry matrix is achieved within minutes by simply adding water. Sample is ready for any downstream analysis without need for any additional purification step.

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Storage/Stability

DNAstable Plus reagent can be stored at 4°C to 20°C until ready for use. The sample pouch allows for dry storage even in uncontrolled humidity environments and serves as an alternative to storage in a dry storage cabinet. The moisture barrier foil bag should be opened just before use.

Procedures

Notes on Sample Preparation

Purification Techniques — Most standard molecular biology techniques and/or commercially available kits are compatible with DNAstable Plus. Purified DNA that is DNase-free and resuspended in water or a low salt buffer can be used directly with DNAstable Plus.

Determining Yield — The concentration of the DNA sample should be determined prior to sample application into DNAstable Plus. Although not essential, applying a known amount of DNA into DNAstable Plus for storage can facilitate sample retrieval and subsequent applications. For optimal results, store between 1 ng and 250 μg of total DNA per tube in liquid format, or up to 50 μg in dry format.

Sample Drying and Storage

  1. Add 25% of the reagent to the DNA sample (≤250 μg), stored in water or any aqueous buffer (e.g., 25 μl of DNAstable Plus to 100 μl of DNA).
  2. Mix the sample thoroughly with gentle pipetting. Avoid forming air bubbles.
  3. Store at room temperature for up to a year, or proceed to step 4.
  4. Dry the uncovered sample completely at room temperature (15-25°C). We recommend using a laminar flow hood or drying under a vacuum concentrator to ensure complete drying (see tables 1 and 2 below for drying times).

Table 1 – drying times in a laminar flow hood

Sample Volume (μl) Drying Times (hrs)
Tubes

Drying Times (hrs)
(96-well plate)
Drying Times (hrs)
(384-well plate)
5 4 4 8
6–10 6 6 12
11–20 12 8 24
21–50 28 18 48
51–100 56 24 68
101–125 72 24 78

 

Table 2 – drying times in a vacuum concentrator at 25-30°C

Sample Volume (μl) Drying Times (hrs)
Tubes
Drying Times (hrs)
(96-well plate)
Drying Times (hrs)
(384-well plate)
5 10 15 80
6–10 15 15 120
11–20 30 30 180
21–50 45 90 360
51–100 60 60 N/A
101–125 75 180 N/A

 

  1. Cover and store samples at room temperature protected from moisture. After complete sample drying, plates should be re-sealed with aluminum foil seals.

Drydown Sample Recovery

DNA stored dry in DNAstable Plus can be recovered by the addition of water or aqueous buffer. Samples are ready for downstream applications without the need for further purification.

  1. Add water or aqueous buffer directly to the dried sample in DNAstable Plus tube or well, to the same volume as original dried down sample. Individual wells in a plate can be opened by puncturing the aluminum foil seal with a pipette tip or razor blade. Samples may be rehydrated directly with aqueous buffers used for downstream applications such as restriction enzyme buffers, PCR buffers, etc.
  2. Incubate at room temperature for 15 minutes to allow complete rehydration.
  3. Mix the sample by gently pipetting up and down. Avoid forming bubbles. The rehydrated sample is now ready for use in downstream applications.

Materials

     
Related Links