Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)

This assay protocol is suitable for the colorimetric detection of Glucose-6-Phosphate (G6P) in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples using the Glucose-6-Phosphate Assay Kit (MAK014).  G6P concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6Ppresent. This kit has a linear range of detection between 0.2–1.0 nmole.

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.  

Reagents

Glucose-6-Phosphate Assay Buffer                   25 mL

Catalog Number MAK014A

Glucose-6-Phosphate Enzyme Mix                       1 vl

Catalog Number MAK014B

Glucose-6-Phosphate Substrate Mix                     1 vl

Catalog Number MAK014C

Glucose-6-Phosphate Standard, 10 mmole           1 vl

Catalog Number MAK014D

Reagents and Equipment Required but Not Provided:

96 well flat-bottom plate – It is recommended to use clear plates (Catalog Number M4436 or equivalent) for colorimetric assays.

Spectrophotometric multiwell plate reader

10 kDa Molecular Weight Cut-Off (MWCO) Spin Filter (Catalog Number Z706345 or equivalent)