Orchid Culture Media – Plant Tissue Culture Protocol

 

Component (mg/L) K4003 P0931 P1056 P6668 P6793 M5524*
Aluminum chloride • 6H2O            
Ammonium nitrate   825.0 825.0 825.0 825.0 1650.0
Ammonium phosphate monobasic            
Ammonium sulfate 500.0          
Boric acid 0.056 3.1 3.1 3.1 3.1 6.2
Calcium chloride anhydrous   166.0 166.0 166.0 166.0 332.2
Calcium nitrate 694.4          
Calcium phosphate tribasic            
Cobalt chloride • 6H2O   0.0125 0.0125 0.0125 0.0125 0.025
Cupric sulfate • 5H2O 0.0624 0.0125 0.0125 0.0125 0.0125 0.025
Na2-EDTA   37.24 37.24 37.24 37.24 37.26
ZnNa2-EDTA            
Ferric chloride • 6H2O            
Ferric citrate            
Ferric sulfate            
Ferric tartrate • 2H2O            
Ferrous sulfate • 7H2O 25.0 27.85 27.85 27.85 27.85 27.8
Magnesium sulfate 122.125 90.35 90.35 90.35 90.35 180.7
Manganese chloride • 4H2O            
Manganese sulfate • H2O 5.682 8.45 8.45 8.45 8.45 16.9
Molybdenum trioxide 0.016          
Molybdic acid (sodium salt) • 2H2O   0.125 0.125 0.125 0.125 0.25
Nickel chloride • 6H2O            
Nickel sulfate • 6H2O            
Potassium chloride            
Potassium iodide   0.415 0.415 0.415 0.415 0.83
Potassium nitrate   950.0 950.0 950.0 950.0 1900.0
Potassium phosphate monobasic 250.0 85.0 85.0 85.0 85.0 170.0
Potassium sulfate            
Sodium nitrate            
Sodium phosphate monobasic            
Sodium sulfate            
Zinc nitrate • 6H2O            
Zinc sulfate • 7H2O 0.331 5.3 5.3 5.3 5.3 8.6
Organics (mg/L) K4003 P0931 P1056 P6668 P6793 M5524*
Activated charcoal     2000.0 2000.0    
Adenine hemisulfate            
Agar            
Banana powder (50% malto-dextrin)     30,000      
6-Benzylaminopurine (BA)         2.0  
D-Biotin            
6gamma-gamma-
Dimethylallylaminopurine
           
Glycine (free base)            
Indole-3-acetic acid            
Indole-3-butyric acid            
Kinetin            
MES (free acid)   1000.0 1000.0 1000.0 1000.0  
myo-Inositol   100.0 100.0 100.0 100.0  
alpha-Naphthaleneacetic acid         0.5  
Nictotinic acid (free acid)   1.0 1.0 1.0 0.5  
Peptone   2000.0 2000.0 2000.0 2000.0  
Pyridoxine • HCl   1.0 1.0 1.0 0.5  
Sucrose 20,000 20,000 20,000 20,000 20,000  
Thiamine • HCl   10.0 10.0 10.0 1.0  
  K4003 P0931 P1056 P6668 P6793 M5524*
Grams of powder to prepare 1 L 21.6 25.3 57.3 27.3 25.3 4.3
pH ± 0.5 at room temperature 4.7 5.0 5.2 5.4 5.0 3.9

*Composition for M5524, Murashige and Skoog Basal Salt Mixture, listed for purposes of comparison only.

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