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Experienced User Protocol
GenElute™ Plant Genomic DNA Miniprep Kit (G2N10, G2N70, G2N350)

For experienced users, an abbreviated protocol is listed below.  For data, images, troubleshooting and other information, please see the full length protocol page.

1. Prepare Plant Tissue - Grind in liquid nitrogen.

2. Release DNA-  Lyse, precipitate, and spin.

3. Filter Lysate - Spin 1 minute.

4. Prepare Column - Add solution and spin.

5. Bind DNA -  Bind and spin twice.

6. Wash Column - Wash and spin twice.

7. Elute DNA - Elute and spin twice.

Storage and Stability

Store the kit at room temperature. If any kit reagent forms a precipitate upon storage, warm at 55–65 °C until the precipitate dissolves.

Precautions and Disclaimer

The GenElute Plant Genomic DNA Miniprep Kit is for laboratory use only. Not for drug, household or other uses. The Lysis Solution [Part A] and the Binding Solution contain guanidine thiocyanate, which is harmful. The Column Preparation Solution is an irritant. Avoid contact with skin. Wear gloves, safety glasses, and suitable protective clothing when handling these solutions or any reagent provided with the kit. Please consult the Materials Safety Data Sheet (MSDS) for information regarding hazards and safe handling practices.

Materials

     
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