Restriction Enzyme Cloning Manual

Molecular Cloning is a set of techniques that are used by molecular biologists to insert a gene-of-interest into a vector capable of replication within the target cell. Once the gene is being expressed in the cells, the system can then be used for a number of different research applications and studies.

An early method that is still of widespread use is restriction enzyme cloning. The method is fairly inexpensive and quite reliable, although it does require some forethought as the researcher is engineering something new and specific to them, for their independent use. The Restriction Enzyme Cloning Manual, provided by Oxford Genetics, aims to assist you through the basic protocols to build and test your expression vector.

Sigma has also developed other resources you may find helpful: 
Molecular Biology Handbook - introduces classic molecular biology techniques (for bacteria, unless otherwise noted). Through each workflow, we provide background information, protocols, references to peer-reviewed literature, and help selecting reagents for your application.

Synthetic Biology Resources - a collection of articles, posters and reviews. Also find links to the synthetic biology community and some great free tools.

Vector choices don’t have to be complicated. Using SnapFast™ Technology built into expression vectors by Oxford Genetics, you get a new standardized tool for your molecular biology toolkit. Exchange DNA components with simple restriction digests to build the plasmid you need for your research. Everything you need for your molecular cloning work is available at Sigma Life Science.

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

About Oxford Genetics

Oxford Genetics specialises in the production of modular DNA plasmids for biological research. Their main technology (SnapFast) is designed to make genetic engineering cheaper and more efficient by providing scientists with a versatile cloning platform and access to a library of matched DNA inserts. Our concept is simple: One plasmid, a thousand possibilities.