Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

5-Bromo-2′-deoxyuridine Protocol

Product No. 10280879001

Protocol

In vivo labeling of rat CNS

Roche does not have data about the in vivo labeling of neuronal cells in rats. The exact concentration of BrdU and the duration of treatment will be dependent on the specific experimental question, e.g., the exact cell type that should be detected, as well as the use of other exogenous compounds. Here are three examples of treatment protocols for labeling of rat neuronal cells, in scientific publications:

  1. D. Charytoniuk, E. Traiffort, P. Hantraye, J. M. Hermel, A. Galdes, and M. Ruat (2002). Intrastriatal sonic hedgehog injection increases Patched transcript levels in the adult rat subventricular zone. European Journal of Neuroscience: Vol 16, p 2351.
  2. I. Jean , C. Lavialle , A. Barthelaix-Pouplard, and C. Fressinaud (2003). Neurotrophin-3 specifically increases mature oligodendrocyte population and enhances remyelination after chemical demyelination of adult rat CNS. Brain Research: Vol 972, p 110.
  3. H. Ohmori, H. Ogura, M. Yasuda, S. Nakamura, T. Hatta, K. Kawano, T. Michikawa, K. Yamashita and K. Mikoshiba (1999). Developmental Neurotoxicity of Phenytoin on Granule Cells and Purkinje Cells in Mouse Cerebellum. Journal of Neurochemistry: Vol. 72, p 1497.

Materials

     
Related Links