G-418 Solution Protocol

Product No. G418-RO

Protocol

OPTIMAL WORKING CONCENTRATION OF G-418 SOLUTION

In this Biochemica article, the dose-response curve of different concentrations of G-418 solution were tested for their ability to stop growth of the adherent PC-3 prostate cancer cell line. Please also refer to the package insert.

ANTIBIOTICS IN A TRANSFECTION PROTOCOL

For best results, do not use antibiotics in a transfection procedure. If needed, they can be added later, at least 4-6 hours after transfection.

USE OF A SCINTILLATION COUNTER INSTEAD OF A LUMINOMETER FOR THE ASSAY

In general, as an alternative to measurement by a luminometer a liquid scintillation counter can be used to detect the signals from the chemiluminescent substrate of the SEAP Reporter Gene Assay, but with a lower sensitivity.
When using a scintillation counter, you have to turn off the coincident circuit to be able to measure chemiluminescence directly (single photon counting mode; e.g., when using a Beckman LS-6000SC liquid scintillation counter use the measuring mode ′Single Photon Monitor′). For further instructions, please contact the manufacturer of the Beckman instrument.

Materials

     
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