CRISPR Webinars

 SygRNA™-Synthetic Two Part CRISPR RNA System – November 16, 2016

The CRISPR/Cas genome editing system has revolutionized most every aspect of the life science industry. Until recently, the most used formats for this technology have been plasmids, mRNA, or lentivirus. Each reagent has been successful in its own right; however, each approach has limitations. SygRNA™, the two-part synthetic crRNA and tracrRNA, increases the pace of research, decreases costs, and can be used with Cas9 protein, Cas9 mRNA and Cas9 expressing cells/models.

This webinar discusses the development of the SygRNA™ system, protocol optimization, and proposes workflows that enable scientists to quickly incorporate CRISPR technologies into their research.

What you will learn:

  • SygRNA™ is a cost effective, fast and efficient tool for gene editing.
  • SygRNA™ alleviates issues associated with random integration and observed stress/toxicity.
  • SygRNA™ removes the need for cloning and careful promoter choice.
  • SygRNA™ negates post IVT sgRNA sequencing.
  • SygRNA™ can be used with multiple formats of Cas9 protein.
  • SygRNA™ can be chemically modified.
  • SygRNA™ production can be scaled up easily.

 Knockout Screening with Sanger Arrayed Genome-Wide CRISPR Libraries – May 12, 2016

 Designing the Next Phase in Genome Editing: Advanced CRISPR Applications - March 1, 2016

CRISPR Cas9 nucleases have revolutionized the field of genome editing enabling unprecedented efficiency of gene targeting in a vast array of cell types and organisms. Even with such a powerful technology at hand, researchers new to the field may find genome modification to be challenging and time-consuming. As CRISPR becomes a focus of the molecular biology research community, MilliporeSigma seeks to share the best approaches learned and methods applied in our years of genome editing experience. Today’s presentation will focus on practical applications of CRISPR for pristine genome editing to achieve knockout as well as specific sequence changes to include donor-mediated snps, reporter-tags and conditional knockouts. Special attention will be paid to design considerations for the donor constructs necessary to achieve specific sequence changes. Finally, the frontiers of CRISPR technology, including synthetic crRNA to fast-track genome editing experiments, whole genome screening and targeted gene activation will be explored.

 Sigma Pooled CRISPR Screening for Functional Genomics - November 10, 2015

This 30 minute webinar will describe the design and application of pooled CRISPR libraries. A Sigma-Aldrich technology expert will discuss unique features of Sigma CRISPR pooled libraries with special attention to CRISPR design and cell culture needs.

Topics will include:

  • Introduction to CRISPR pooled screening concepts.
  • Suggestions for preparatory experiments to help ensure successful screening.
  • Practical considerations for appropriate scaling of cell culture experiments
  • Lentiviral vector formats for delivering CRISPR components
  • Cell enrichment via antibiotic and FACS methods.
  • Counting sgRNA changes to discover new biology: statistical considerations for quantification via deep sequencing.
  • Comparison of CRISPR options for pooled genome editing and pooled transcriptional regulation.

 Sigma CRISPR Paired Nickases for Genome Editing - October 28, 2014

This 30 minute webinar will describe the function, design and application of CRISPR paired nickases. A Sigma-Aldrich technology expert will discuss unique features of CRISPR paired nickases with special attention to specificity and design density critical to genome editing experiments.

Topics will include:

  • Introduction to CRISPR paired nickase
  • Enhanced CRISPR target specificity of Cas9D10A
  • Design density in small target regions with paired nickase
  • Integration of donor constructs using paired nickase
  • CRISPR paired nickase as a tool to introduce disease-relevant single nucleotide polymorphisms (snps)

 Practical Utilization of CRISPR in the Laboratory Workflow - April 22, 2014

This 40 minute webinar will focus on the practical laboratory techniques needed to create and validate genetically modified cell lines using CRISPR. A Sigma-Aldrich technology expert will discuss guidelines and techniques utilized when performing genome editing experiments.

Topics will include:

  • Transfection of CRISPR reagent into a cell line
  • How to validate the activity level of CRISPR
  • Clonal isolation
  • How to screen clonal populations based on desired modification

 Genome Editing with CRISPR Systems - January 14, 2014

CRISPR endonucleases have recently taken the research world by storm with their elegant and simple mode of RNA-guided gene targeting. Watch our free educational webinar below to learn how CRISPR endonucleases can operate via protocols developed for ZFN-based genome editing in human cells, rats, flies, worms, and plants (to name only a few).