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biological source
mouse
Quality Level
clone
CC-1, monoclonal
form
liquid
contains
≤0.1% sodium azide
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
technique(s)
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (frozen sections): suitable
dilution
(Free-floating SectionsParaffin Sections)
isotype
IgG2b
shipped in
wet ice
storage temp.
2-8°C
General description
Recognizes APC in oligodendrocytes and astrocytes.
- Antibody Target Gene Symbol: APC
- Target Synonym: AI047805, Apc7, AU020952, AW124434, BTPS2, DP2, DP2.5, DP3, Familial adenomatous polyposis, FAP, GS, Min, RATAPC
- Entrez Gene Name: adenomatous polyposis coli
- Hu Entrez ID: 324 (Related Antibodies: ST1150, OP62, OP47L, OP44)
- Mu Entrez ID: 11789
- RatEntrez ID: 24205
Immunogen
a recombinant protein consisting of amino acids 1-226 of APC
Application
Frozen Sections (5 µg/ml) Immunofluorescence (5 µg/ml) Paraffin Sections (5 µg/ml, heat pre-treament required) Immunocytochemistry (see application references) Free Floating Sections (see application references)
Well suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody′s staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.
Well suited for IHC and IF studies of oligodendrocytes and optic nerves due to the antibody′s staining of the cell body as opposed to the myelinated processes. Both frozen sections and formalin-fixed, paraffin-embedded brain sections can be used. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration
Warning
Toxicity: Standard Handling
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Negative control: MOPC 21 cells
Positive control: Purkinje cells or cerebellum tissue
Positive control: Purkinje cells or cerebellum tissue
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J Groden et al.
Cell, 66(3), 589-600 (1991-08-09)
DNA from 61 unrelated patients with adenomatous polyposis coli (APC) was examined for mutations in three genes (DP1, SRP19, and DP2.5) located within a 100 kb region deleted in two of the patients. The intron-exon boundary sequences were defined for
L K Su et al.
Science (New York, N.Y.), 262(5140), 1734-1737 (1993-12-10)
Mutations of APC appear to initiate sporadic and inherited forms of human colorectal cancer. Although these mutations have been well characterized, little is known about the function of the APC gene product. Two cellular proteins that associate with APC were
R V Bhat et al.
Glia, 17(2), 169-174 (1996-06-01)
Mutations of the adenomatous polyposis coli (APC) tumor suppressor gene have been linked to familial polyposis, an inherited predisposition to colon cancer, and a high percentage of sporadic colon adenomas. Although this gene is best known for its role in
K W Kinzler et al.
Science (New York, N.Y.), 251(4999), 1366-1370 (1991-03-15)
Recent studies have suggested the existence of a tumor suppressor gene located at chromosome region 5q21. DNA probes from this region were used to study a panel of sporadic colorectal carcinomas. One of these probes, cosmid 5.71, detected a somatically
The APC gene product in normal and tumor cells
Smith, K.J., et al.
Proceedings of the National Academy of Sciences, 90, 2846-2850 (1993)
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