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M2279

Sigma-Aldrich

Minimum Essential Medium Eagle

With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
MEM
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

application(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

Earle’s salts (5% CO2): yes
NaHCO3: yes
phenol red: yes
HEPES: no
sodium pyruvate: no
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Qingyun Li et al.
Neuron, 101(2), 207-223 (2019-01-05)
Microglia are increasingly recognized for their major contributions during brain development and neurodegenerative disease. It is currently unknown whether these functions are carried out by subsets of microglia during different stages of development and adulthood or within specific brain regions. Here...
Winston X Yan et al.
Nature communications, 8, 15058-15058 (2017-05-13)
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along the genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ and Sequencing (BLISS)...
Luciano A Masullo et al.
Nature communications, 9(1), 3281-3281 (2018-08-18)
The theoretically unlimited spatial resolution of fluorescence nanoscopy often comes at the expense of time, contrast and increased dose of energy for recording. Here, we developed MoNaLISA, for Molecular Nanoscale Live Imaging with Sectioning Ability, a nanoscope capable of imaging...
Hiroaki Mitsuhashi et al.
Biology open, 7(4) (2018-04-06)
Aberrant expression of the full-length isoform of DUX4 (DUX4-FL) appears to underlie pathogenesis in facioscapulohumeral muscular dystrophy (FSHD). DUX4-FL is a transcription factor and ectopic expression of DUX4-FL is toxic to most cells. Previous studies showed that DUX4-FL-induced pathology requires...
Optimized low-dose combinatorial drug treatment boosts selectivity and efficacy of colorectal carcinoma treatment.
Zoetemelk, et al.
Molecular Oncology, 14, 2894-2919 (2021)

Articles

MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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