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RPMI-1640 Medium

With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

MDL number:

Quality Level






cell culture | mammalian: suitable


endotoxin, tested


phenol red: yes
sodium pyruvate: no
L-glutamine: no
NaHCO3: yes

shipped in


storage temp.


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RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.


Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.


Supplement with 0.3 g/L L-glutamine.

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine.


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