Large molecule HPLC

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Showing 1-30 of 74 results

Quantification of Infliximab in Human Serum by LC-MS/MS Using A Full-Length Stable Isotope Labeled Internal Standard

Infliximab, a chimeric monoclonal antibody, is used to treat rheumatoid arthritis, psoriatic arthritis, Crohn’s disease, and other autoimmune diseases.

Oligonucleotide Quality Control by Analytical HPLC

In many cases oligonucleotides require quantitative analysis to verify specifications have been met. MALDI-TOF and ESI-MS can provide qualitative-quantification for purity.

Peptide Sample Amount Determination

Determining the amount of desired product in a peptide sample.

HPLC Tips & Tricks: Increase Your HPLC/UHPLC Method Sensitivity

Tips & tricks to improve your chromatographic method sensitivity by reducing baseline noise and/or making suggested improvements to increase signal intensity.

Biopolymer Separations

Supelco’s product offering for biopolymer separations includes columns and media categorized by separation mode, as well as by column brand.

Ascentis® Express F5 (Pentafluorophenyl) U/HPLC Columns

Enhanced selectivity for stereoisomers and closely related compounds with alternate selectivity from C18 phases for basic, acidic, or neutral compounds

Ascentis® Express Biphenyl UHPLC and HPLC Columns

The bonded, densely endcapped, dimethyl-biphenyl stationary phase of Ascentis® Express 90 Å Biphenyl, based on fused-core particles, provides a stable, reversed phase packing with enhanced π-π and mild steric interactions due to the two sequential phenyl groups bonded to the

BIOshell™ A400 Protein C4 U/HPLC Columns

BIOshell™ A400 Protein C4 columns contain 3.4 μm particles with 400 Å pores, which are derivatized with dimethylbutyl silane and exhaustively endcapped for optimum protein recovery.

Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improve your reversed phase selectivity for polar aromatics and heterocyclic compounds with the Ascentis® Express Phenyl-Hexyl U/HPLC column.

Ascentis® Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.

Chromatography Profile Analysis of Phosphorothioate Oligonucleotides

The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular

HPLC Columns for Antibody and Large Protein Analysis

BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.

HPLC Tips & Tricks: Mobile Phase Preparation Part 2 - Buffers

Learn the tips and tricks of selecting and preparing a suitable mobile phase buffer for your HPLC separations and method developments for samples that contain ionizable compounds.

Optimising the LC-MS Analysis of Biomolecules

We present an article concerning optimising the LC-MS analysis of biomolecules.

BIOshell™ Glycan U/HPLC Columns

The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.

Ascentis® Express RP-Amide Columns for HPLC and UHPLC

Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.

Developing HPLC Methods

We recommend every HPLC method developer have these four columns in their arsenal; Ascentis C18, Ascentis RP-Amide, Ascentis Phenyl and Discovery HS F5.

SupraSolv® GC Solvent Specifications

Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.

HPLC Tips & Tricks: Optimizing Injection Volume

Learn the tips and tricks to optimize the injection volume during your High-Performance Liquid Chromatography (HPLC) method-based analyses and further, how it affects your peak area.

Ascentis ® Express HILIC U/HPLC Columns

For separation of polar compounds including polar neutrals, polar acids, and polar and non-polar basic amines use our Ascentis® Express HILIC column.

Fast and high-resolution Analysis of Intact Therapeutic Monoclonal Antibody Trastuzumab using BIOshell™ A400 Protein C4 Column

Optimization of a Reversed-Phase Liquid Chromatographic (RP-LC) method for the intact analysis of a therapeutic monoclonal antibody, trastuzumab, using a BIOshell™ A400 Protein C4 column.

Released N-Glycan Analysis of a Therapeutic Antibody Using BIOshell™ Glycan Column

This application note describes the released N-Glycan analysis of a monoclonal antibody, cetuximab, labeled with procainamide, using a BIOshell™ Glycan HPLC column.

Supelco Preparative HPLC products for Pharmaceutical Development and Production

HPLC Tips & Tricks – Mobile Phase Preparation

Tips and tricks to prepare the correct and consistent mobile phase for optimized separations during your High-performance Liquid Chromatographic (HPLC) based method developments and optimizations.

UHPLC-MS Bottom-Up Analysis of Trastuzumab on a BIOshell™A160 Peptide C18 Column

Learn about UHPLC-MS analysis of trastuzumab on a BIOshell™ 160A peptide C18 column and discover reduced solvent consumption in peptide mapping using 1.5 mm I.D. columns.

Monoclonal Antibody Titer Determination Using a Chromolith® WP 300 Protein A Wide Pore Monolithic Silica HPLC Column

Method development for titer determination of three different monoclonal antibodies - cetuximab, trastuzumab, and universal antibody standard using a Chromolith® WP 300 Protein A column.

Fast Analysis of Monoclonal Antibodies Using Silica Monoliths Designed for Bioanalysis

Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.

Ascentis^® Express C18 U/HPLC Columns

Ascentis® Express C18 is the first choice when starting a new separation method.

Middle Up Mass Analysis Using Trastuzumab as Model Protein

A RPLC-MS workflow for mass analysis of reduced monoclonal antibody, trastuzumab, including reduction procedures, calibration, and system suitability tests.

A RPLC-MS workflow for mass analysis of non-reduced monoclonal antibody, trastuzumab, including calibration, and system suitability tests.

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