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[Chemical constituents of Rhododendron seniavinii].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2013-05-15)
Qing-Qing Wang, Ying Zhang, Wen-Cai Ye, Guang-Xiong Zhou
ABSTRACT

To study the chemical constituents of Rhododendron seniavinii. Compounds were isolated from the aqueous extract of the leaves of R. seniavinii by using Sephadex LH-20, ODS open column chromatography and other means. Their structures were elucidated according to spectral data and physiochemical properties. Thirteen compounds were isolated from R. seniavinii and identified as 5-methoxydehydroconiferyl alcohol (1), dehydroconiferyl alcohol (2), (-)-syringaresinol (3), (-)-lyoniresinol (4), (+)-lyoniresinol 3alpha-O-beta-D-glucopyranoside (5), (-)-lyoniresinol 3alpha-O-beta-D-glucopyranoside (6), 3,4,5-trimethoxyphenyl-1-O-beta-D-glucopyranoside (7), nikoenoside (8), 3,5,7-trihydroxychromone-3-0-alpha-L-rhamnopyranoside (9), 3,4,5-trimethoxyphenol (10), scopoletin (11), scopolin (12) and quercitrin (13). Compounds 1-12 were obtained from this plant for the first time.

MATERIALS
Product Number
Brand
Product Description

Quercitrin, primary reference standard
Supelco
Scopoletin, analytical standard
Sigma-Aldrich
Sephadex® G-50, Superfine
Sigma-Aldrich
Sephadex® G-10, Medium
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Sephadex® G-50, Fine
Supelco
Sephadex® G-50, Medium
Sigma-Aldrich
Sephadex® G-50, DNA grade, BioReagent, Molecular Biology, medium
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Sephadex® G-50, BioReagent, DNA grade, Molecular Biology, fine
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Scopoletin, ≥99%
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Dextran from Leuconostoc spp., Mr ~40,000
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Sephadex® G-75, Medium
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Dextran from Leuconostoc mesenteroides, average mol wt 150,000
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Dextran from Leuconostoc mesenteroides, average mol wt 15,000-30,000
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Dextran from Leuconostoc mesenteroides, average mol wt 1,500,000-2,800,000
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Dextran from Leuconostoc mesenteroides, average mol wt 35,000-45,000
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Sephadex® G-75, Superfine
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Sephadex® G-25, Coarse
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Dextran, enzymatic synth.
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Dextran from Leuconostoc spp., Mr ~70,000
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Dextran from Leuconostoc mesenteroides, Mr ~60,000
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Dextran from Leuconostoc spp., Mr 450,000-650,000
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Dextran from Leuconostoc mesenteroides, Mr ~200,000
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Sephadex® G-100, Superfine
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Dextran from Leuconostoc spp., Mr ~6,000
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Sephadex® G-15, Medium
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Sephadex® G-100, Medium
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Sephadex® G-25, Medium
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Sephadex® G-25, Fine
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Dextran solution from Leuconostoc mesenteroides, 20 % (w/w) (Autoclaved)
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Dextran from Leuconostoc spp., Mr ~100,000