HomeMILLIPLEX® Multiplex for Luminex® ImmunoassaysTips & Tricks Guide to MILLIPLEX® Multiplexing

Tips & Tricks Guide to MILLIPLEX® Multiplexing

We’re so confident in the benefits of MILLIPLEX® multiplex kits that we’ve compiled tips and tricks, straight from the experts, to eliminate any doubt in your ability to multiplex like a pro. Enhance the power of your research with MILLIPLEX® multiplexing by learning more about industry guidance, multiplexing tips, one-day multiplex assays for quick turnaround times, and more.

Industry Guidance for Immunoassays

Let industry guidance lead you to MILLIPLEX® multiplexing. From academia to contract research to pharma, MILLIPLEX® kits meet the ever-increasing demand for high-quality immunoassays for reproducible results. This includes:

  • Detection and sensitivity
  • Performance in a sample matrix
  • Specificity
  • Selectivity
  • Precision and accuracy
  • Linearity
  • Stability
  • Cross-talk
  • Lot-to-lot reproducibility
  • Vendor support

Want to learn more about industry guidance on assay development and validation? We recommend these references1,2,3 below.

One-Day MILLIPLEX® Multiplex Assays for Limited Lab Time

Many of our MILLIPLEX® multiplex assays can be completed in a single day by using a shorter primary incubation time. With only 90 minutes of hands-on time required4, these assays allow for more time to work on other important laboratory projects. Results are available in 4-5 hours, depending on incubation times, making them ideal for limited laboratory time.

The typical MILLIPLEX® one-day assay workflow is as follows:

  • Step 1: Set up plate with samples/standards, beads, buffer/matrix. Incubate at room temperature for 1-2 hours*.
  • Step 2: Wash plate. Add detection antibodies. Incubate at room temperature for 1 hour*.
  • Step 3: Wash plate*. Add Streptavidin-Phycoerythrin. Incubate at room temperature for 30 minutes.
  • Step 4: Wash plate. Read on Luminex® instrument.

*See protocol for specific incubation times and wash steps.

More than 50 MILLIPLEX® multiplex kits, covering several research areas, are available with a single-day protocol. This includes more than 30 immunology assays, as well as virology, metabolism/endocrinology, cardiovascular, neuroscience, and more. MILLIPLEX® multiplex assays spanning multiple species can be completed in one day, including human, mouse, rat, non-human primate, and many of our veterinary medicine and animal health panels. Select single-day assays are included below.

Immunology/Inflammation Assays
Virology Assays
Metabolism/Endocrinology Assays
Cardiovascular Assays
Bone Assays
Neuroscience Assays
Toxicity Assays

For Research Use Only. Not For Use In Diagnostic Procedures.

Request Information

Have more questions about MILLIPLEX® multiplex assays? Fill out the form by clicking on the button below.


Lee JW, Devanarayan V, Barrett YC, Weiner R, Allinson J, Fountain S, Keller S, Weinryb I, Green M, Duan L, et al. 2006. Fit-for-Purpose Method Development and Validation for Successful Biomarker Measurement. Pharm Res. 23(2):312-328.
Jani D, Allinson J, Berisha F, Cowan KJ, Devanarayan V, Gleason C, Jeromin A, Keller S, Khan MU, Nowatzke B, et al. 2016. Recommendations for Use and Fit-for-Purpose Validation of Biomarker Multiplex Ligand Binding Assays in Drug Development. AAPS J. 18(1):1-14.
Andreasson U, Perret-Liaudet A, van Waalwijk van Doorn LJC, Blennow K, Chiasserini D, Engelborghs S, Fladby T, Genc S, Kruse N, Kuiperij HB, et al. A Practical Guide to Immunoassay Method Validation. Front. Neurol.. 6
Günther A, Becker M, Göpfert J, Joos T, Schneiderhan-Marra N. Comparison of Bead-Based Fluorescence Versus Planar Electrochemiluminescence Multiplex Immunoassays for Measuring Cytokines in Human Plasma. Front. Immunol.. 11
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?