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Merck

D9170

Sigma-Aldrich

Dimetilsulfóxido

PCR Reagent

Sinónimos:

DMSO

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1 AMP
US$ 50,00
5 AMP
US$ 182,00

US$ 50,00

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Fórmula lineal:
(CH3)2SO
Número CAS:
Peso molecular:
78.13
Beilstein/REAXYS Number:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.25
Assay:
≥99.5%
Technique(s):
PCR: suitable
Application(s):
agriculture
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

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vapor density

2.7 (vs air)

Quality Level

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.5%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

packaging

vial of 1 mL

technique(s)

PCR: suitable

impurities

≤0.1% water
water

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

suitability

suitable for PCR

application(s)

agriculture

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Este artículo
34869D8418D5879
application(s)

agriculture

application(s)

food and beverages

application(s)

-

application(s)

-

grade

PCR Reagent

grade

-

grade

for molecular biology

grade

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

assay

≥99.5%

assay

≥99.7%

assay

≥99.9%

assay

≥99.5%

technique(s)

PCR: suitable

technique(s)

HPLC: suitable

technique(s)

DNA sequencing: suitable, PCR: suitable, transfection: suitable

technique(s)

immunoblotting: suitable

solubility

H2O: miscible (completely)

solubility

-

solubility

H2O: miscible (completely)

solubility

H2O: soluble(lit.), acetone: soluble(lit.), benzene: soluble(lit.), chloroform: soluble(lit.), ethanol: soluble(lit.)

General description

Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs).[1] Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination.[2] As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.[3]

Application

Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture[4] and as PCR facilitator.[2][5]

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Disclaimer

Fácilmente superenfriado, vuelve a fundir lentamente a temperatura ambiente. Producto solidificado que puede volver a licuarse por calentamiento a temperatura ambiente sin detrimento del producto.

Clase de almacenamiento

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Questions

1–9 of 9 Questions  
  1. How much DMSO is in each of the vials (D9170)?

    1 answer
    1. As stated in the 'PROPERTIES' section, each vial contains 1 mL. One ampule contains 1 mL of Dimethyl Sulfoxide, and 5 amps include 5 ampules containing 1 mL of Dimethyl Sulfoxide. each.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Where does DMSO come from?

    1 answer
    1. DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. What is the molarity of DMSO?

    1 answer
    1. DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

      Helpful?

  7. What is the DMSO dissolved in?

    1 answer
    1. DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

      Helpful?

  8. Does DMSO have any synthetic utility?

    1 answer
    1. DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

      Helpful?

  9. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    1 answer
    1. DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

      Helpful?

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