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Ultrafiltration/Diafiltration (UF/DF) in Plasmid DNA Manufacturing

How are ultrafiltration and diafiltration used in plasmid DNA manufacturing and how should you optimize this process? Learn more in our technical article and download our free eBook.

Viral Safety in Bioprocessing

Viral safety in bioprocessing relies on a framework of (1) preventing contamination, (2) detecting contamination, and (3) removing or inactivating viral contaminants.

Tangential Flow Filtration in the Antibody Drug Conjugate (ADC) Manufacturing Process

Find best practices for using Pellicon® Capsules in ADC processing as well as data from two studies using Pellicon® Capsules and Pellicon® cassettes in the ADC manufacturing process.

Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production

Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.

Bioburden Control in Bioprocessing

Bioburden control in bioprocessing requires assessing the risks, mitigating the risks, and monitoring bioburden levels.

Viscosity Reduction in High Concentration TFF

This overview describes how use of viscosity-reducing excipients can address viscosity challenges in high concentration tangential flow filtration (TFF) and improve process economics.

Membrane Prefilters Improve Sterilizing Filter Capacity and Process Economics in Bioprocessing

Prefilters can be used throughout bioprocessing to remove to remove particulates, improve quality and protect sterilizing filters and downstream unit operations.

Renewable Electricity Toolkit

The Renewable Electricity Supplier Toolkit helps suppliers learn renewable electricity basics and how to bring projects to life.

Solubility and Dissolution Enhancement Via Formulation

Our overview of API solubility and dissolution enhancement methods can help you decide between the many options to take during formulation.

Cell Counting Using a Hemocytometer

Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.

Quantification and Quality Control via CCM Fingerprinting

Learn about rapid and cost-effective identification of more than 100 media formulation components using LC-MS cell culture media fingerprinting.

Sterilizing Grade Filtration Unit Operations for Plasmid DNA Processes

We discuss the final filtration step of plasmid DNA manufacturing and its key considerations and challenges. Download our free pDNA downstream purification eBook or ask our experts.

An Introduction to Horseradish Peroxidase (HRP) and Its Applications

Horseradish peroxidase (HRP) is an enzyme used for detecting molecules, degrading toxins, cross-linking proteins, and in diagnostics like lateral flow assays.

Single-Pass Tangential Flow Filtration Implementation and Scaling

Get practical guidance on using single-pass tangential flow filtration in your downstream process and scaling between capsules and cassettes.

Tangential Flow Filtration in Downstream Bioprocessing

There are many options for tangential flow filtration (TFF) in downstream bioprocessing from multi-use to single-use TFF or batch TFF and single-pass TFF.

Purification of Extracellular Vesicles with Eshmuno^® Q Resin and Natrix^® Q Membrane

Separation and purification of subpopulations of extracellular vesicles (EVs) using Eshmuno® Q anion exchange resin and Natrix® Q membrane chromatography.

Nitrate Levels in Vegetables

Reflectometric nitrate determination in vegetables using Griess reagent and Reflectoquant® system.

Nitrate Detection in Wastewater

Reflectometric determination of nitrate in wastewater using Griess reagent and Reflectoquant® test strips.

Troubleshooting Puriﬁcation Methods

This page shows troubleshooting methods for puriﬁcation of GST-tagged proteins using Cytiva products.

Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins

Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Cell Lysate Preparation

Cell lysis challenges and protein extraction variables for Western blot sensitivity and reproducibility.

Protein Visualization

Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.

How to Choose a Secondary Antibody

Review the key factors that should figure in your decision to choose a secondary antibody. Learn about species, subclass, isotype, label, and more.

Actinin

Introduction about α-Actinin protein present in both muscle and non-muscle cells.

Adenylyl Cyclase

Roles of adenylyl cyclases in cell regulation across different organisms.

α1-Adrenoceptor Characteristics and Modulators

Review alpha 1 adrenoceptors as well as their agonists, antagonists, and tissue expression patterns. We suggest several modulators and alternatives for working with a-1 adrenoreceptors.

Angiotensin Receptors

We offer many products related to angiotensin receptors for your research needs.

Biglycan

Biglycan is a 200-350 kDa(proteoglycan consisting of a 45 kDa core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains.

Bioactive Molecules for Apoptosis

Apoptosis regulation involves multiple pathways and molecules for cellular homeostasis.

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