[Direkt zum Inhalt](https://www.sigmaaldrich.com#main-content) [![Merck](https://www.sigmaaldrich.com/static/logos/purple/merck.svg)](https://www.sigmaaldrich.com/AT/de) Produkte Warenkorb0 ATDE Produkte ProdukteAnwendungenDienstleistungenRessourcenUnterstützung [Anmelden / Registrieren](https://www.sigmaaldrich.com/oidc-sign-in) [Bestellungssuche](https://www.sigmaaldrich.com/AT/de/order-lookup) [Schnelleinkauf](https://www.sigmaaldrich.com/AT/de/quick-order) Warenkorb0 [Home](https://www.sigmaaldrich.com/AT/en)[Sample Purification](https://www.sigmaaldrich.com/AT/en/applications/analytical-chemistry/sample-preparation/purification)Non-volatile and Volatile Buffer Systems # Non-volatile and Volatile Buffer Systems ## Non-volatile buffers for anion exchange chromatography ![Non-volatile buffers for anion exchange chromatography](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/analytical-chemistry/purification/non-volatile-buffers.jpg "Non-volatile buffers for anion exchange chromatography") pH intervalSubstanceConc. (mM)Counter-ionpKa (25 °C)1d(pKa)/ dT (°C) 4.3–5.3N-Methylpiperazine         20Cl-4.75-0.015 4.8–5.8Piperazine 20Cl- or HCOO-5.33-0.015 5.5–6.5L-Histidine20Cl-6.04  6.0–7.0bis-Tris 20Cl-6.48-0.017 6.2–7.2; 8.6–9.6bis-Tris propane 20Cl-6.65; 9.10  7.3–8.3Triethanolamine 20Cl- or CH3COO-7.76-0.020 7.6–8.6Tris 20Cl-8.07-0.028 8.0–9.0N-Methyldiethanolamine 20SO42-8.52-0.028 8.0–9.0N-Methyldiethanolamine 50Cl- or CH3COO-8.52-0.028 8.4–9.4Diethanolamine20 at pH 8.4 50 at pH 8.8Cl-8.88-0.025 8.4–9.4Propane 1,3-Diamino     20Cl-8.88 -0.031 9.0–10.0Ethanolamine 20Cl-9.50-0.029 9.2–10.2Piperazine 20Cl-9.73-0.026 10.0–11.0 Propane 1,3-Diamino       20Cl-10.55-0.026 10.6–11.6Piperidine 20Cl-11.12-0.031 1Ref: Handbook of chemistry and physics, 83rd edition, CRC, 2002–2003. ## Non-volatile buffers for cation exchange chromatography ![Non-volatile buffers for cation exchange chromatography](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/analytical-chemistry/purification/non-volatile-buffers-for-cation.jpg "Non-volatile buffers for cation exchange chromatography") pH intervalSubstanceConc. (mM)Counter-ionpKa (25 °C)1d(pKa)/ dT (°C) 1.4–2.4Maleic acid20Na+1.92  2.6–3.6Methyl malonic acid20Na+ or Li+3.07  2.6–3.6Citric acid20Na+3.13-0.0024 3.3–4.3 Lactic acid50Na+3.86  3.3–4.3Formic acid50Na+ or Li+3.75+0.0002 3.7–4.7; 5.1–6.1 Succinic acid50Na+4.21; 5.64-0.0018 4.3–5.3Acetic acid50Na+ or Li+4.75+0.0002 5.2–6.2 Methyl malonic acid50Na+ or Li+5.76  5.6–6.6MES50Na+ or Li+6.27-0.0110 6.7–7.7 Phosphate50Na+7.20-0.0028 7.0–8.0HEPES50Na+ or Li+7.56-0.0140 7.8–8.8BICINE50Na+8.33-0.0180 1Ref: Handbook of chemistry and physics, 83rd edition, CRC, 2002–2003. ### Volatile buffer systems pH rangBuffer systemCounter-ionpKa-values for buffering ions1 3.3–4.3Formic acid H+3.75 3.3–4.3; 4.8–5.8 Pyridine/formic acidHCOO-3.75; 5.25 3.3–4.3; 9.3–10.3Trimethylamine/formic acid HCOO-4.75; 9.81 4.3–5.8 Pyridine/acetic acidCH3COO-4.75; 5.25 4.3–5.3; 9.3–10.3Trimethylamine/acetic acid CH3COO-4.75; 9.81 3.3–4.3; 8.8–9.8 Ammonia/formic acidHCOO-3.75; 9.25 4.3–5.3; 8.8–9.8Ammonia/acetic acid CH3COO-4.75; 9.25 5.9–6.9; 9.3–10.3 Trimethylamine/carbonateCO32-6.35; 9.81 5.9–6.9; 8.8–9.8Ammonium bicarbonateHCO3-6.35; 9.25 5.9–6.9; 8.8–9.8 Ammonium carbonate/ammoniaCO32-6.35; 9.25 5.9–6.9; 8.8–9.8Ammonium carbonateCO32-6.35; 9.25 4.3–5.3: 7.2–8.2 N-ethylmorpholine/acetateHCOO-4.75; 7.72 1Ref: Handbook of chemistry and physics, 83rd edition, CRC, 2002–2003. ## Materials Bitte entschuldigen Sie, es ist ein unerwarteter Fehler aufgetreten Response not successful: Received status code 500 __Related Articles__ - [Maintenance of Multimodal Chromatography Media and Storage Conditions](https://www.sigmaaldrich.com/AT/en/technical-documents/protocol/protein-biology/protein-purification/maintenance-of-media-and-storage-conditions) - [Selection of Purification Equipment](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/analytical-chemistry/purification/appendix4) - [Bind/Elute vs Flowthrough Mode in Multimodal Chromatography](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/analytical-chemistry/purification/bind-elute-vs-flowthrough-mode-in-multimodal-chromatography) - [Chiral Chromatography Frequently Asked Questions](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/analytical-chemistry/purification/faq) - [FastWoRX for Faster and Greener Alternative to Liquid-Liquid Extraction](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/analytical-chemistry/purification/fastworx) - [High Purity Carbon Adsorbents for Chromatography](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/analytical-chemistry/purification/high-purity-carbon-adsorbents) - [Plasmid purification by ion exchange chromatography with Genopure kits](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/analytical-chemistry/purification/ion-exchange-chromatography) - [LRA (Lipid Removal Agent) for Biopharmaceutical Production](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/analytical-chemistry/purification/lra-lipid-removal) - [View More](https://www.sigmaaldrich.com/AT/en/search/facet-search?focus=sitecontent&term=facet-search) __Related Product Categories__ - [HPLC Buffers](https://www.sigmaaldrich.com/AT/en/products/analytical-chemistry/analytical-chromatography/hplc-buffers) - [Biological Buffers](https://www.sigmaaldrich.com/AT/en/products/chemistry-and-biochemicals/biochemicals/biological-buffers) Oben __Melden Sie sich an, um fortzufahren.__ Um weiterzulesen, melden Sie sich bitte an oder erstellen ein Konto. 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