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[Home](https://www.sigmaaldrich.com/AT/en)[Bioburden Testing](https://www.sigmaaldrich.com/AT/en/applications/microbiological-testing/bioburden-testing)EZ-Fluo ™ Rapid Microbial Detection System
# EZ-Fluo ™ Rapid Microbial Detection System
## Rapid and Quantitative Detection of Microorganisms
EZ-Fluo™ System is a rapid fluorescence-based technology for the early detection of contaminants over a broad range of filterable matrices. The EZ-Fluo™ system uses a non-destructive method to speed up the detection of microbial contamination. Unlike many other rapid test systems, this method allows the identification of microorganisms from the same sample, after they have been stained, using any standard ID technology.
- Rapid results in one-third of the time in comparison to traditional techniques
- Flexible approach based on standard membrane filtration with reduced incubation time on a media plate
- Non-destructive method—compatible with any ID technique
- Compact hardware – fits easily on any laboratory bench
- Cost-effective solution for routine and investigatory use
- [](https://www.sigmaaldrich.com)[EZ-Fluo™ System Applications and Sample Types](https://www.sigmaaldrich.com#sample)
- [Faster Product Release](https://www.sigmaaldrich.com#productrelease)
- [EZ-Fluo™ System Workflow](https://www.sigmaaldrich.com#workflow)
- [EZ-Fluo™ Spot Counter Software](https://www.sigmaaldrich.com#software)
- [Support and Services](https://www.sigmaaldrich.com#support)
- [Related Products](https://www.sigmaaldrich.com#relatedproducts)
[Request More Information](https://www.sigmaaldrich.com/AT/en/campaigns/rapid-microbial-testing-methods-info)
## [](https://www.sigmaaldrich.com)EZ-Fluo™ System Applications and Sample Types
The EZ-Fluo™ system can rapidly detect and quantify microbial contamination in any filterable sample. Potential applications are numerous in pharmaceutical, beverage, and personal care industries for testing the following sample types:
- Raw materials
- In-process samples
- Final products
- Environmental samples
Concrete examples? Check out the following application notes:
[Rapid detection of yeast, mold and bacterial contaminants in beverages using fluorescence-based technology](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/343/384/rapid-microbial-detection-beverages-an1830en00-ms.pdf)
[Rapid detection of microorganisms in a gel-based hand wash using fluorescence-based technology](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/424/096/rapid-microbial-detection-hand-wash-an7058en-ms.pdf)
## [](https://www.sigmaaldrich.com)Faster Product Release
Today, more and more QC laboratories are switching to rapid methods for the detection of microbial contamination to reduce the time to result. Traditional methods require several days to provide results and have limited value when it comes to faster release of products to the market or conducting investigations if necessary.
EZ-Fluo™ Rapid System can detect viable microorganisms filtered on a membrane filter down to 1 CFU per sample. EZ-Fluo™ test results are easily comparable to compendial methods, which facilitates the validation and the integration of this rapid system in any laboratory without causing disruption.
## [](https://www.sigmaaldrich.com)EZ-Fluo™ System - Workflow
The EZ-Fluo™ Rapid Detection System consists of a reader and staining reagents used in combination with filtration membranes and standard culture media.
__Sample preparation:__ The sample contaminants are collected by membrane filtration using the [](https://www.sigmaaldrich.com)[](https://www.sigmaaldrich.com)[EZ-Fit™ Filtration Unit](https://www.sigmaaldrich.com/AT/en/search/ez-fit-filtration-unit?focus=products&page=1&perpage=30&sort=relevance&term=ez-fit%20filtration%20unit&type=product). After a short incubation time, media plates are removed from the incubator. Each membrane filter is then transferred onto a cellulose pad that has been soaked with the EZ-Fluo™ fluorescent staining solution. The membrane filters are further incubated for 30 minutes.
__Micro-colonies enumeration:__ The fluorescent stained colonies can be counted two ways – directly through the viewing window of the EZ-Fluo™ reader or using the EZ-Fluo™ Camera and dedicated software, which projects the image onto a computer screen.
__Re-incubation:__ After reading, membrane filters can be re-incubated onto the culture media for later collection and identification of microorganisms if contamination of the sample was detected.
## [](https://www.sigmaaldrich.com)EZ-Fluo™ Spot Counter Software
The EZ-Fluo™ camera combined with the EZ-Fluo™ Spot Counter software makes the sample reading more convenient and allows you to track records for optimized microbiology QC labs. [__Download the software here.__](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/articles/microbiological-testing/bioburden-testing/ez_flou_spot_counter_software_v1.1.2.zip)
## [](https://www.sigmaaldrich.com)Support and Services
We provide a comprehensive [__range of support and services__](https://www.sigmaaldrich.com/AT/en/services/product-services/maintenance-and-service-plans/microbiology-maintenance-and-service-plans) to help you save your QC resources and facilitate the implementation of the EZ-Fluo™ system into your daily testing routine. Our highly experienced service teams can assist you with the following activities:
- Feasibility study and on-site evaluation
- Method development and method development consultancy
- Equipment installation
- Operator training
- On-site system qualification (IQ/OQ)
- Yearly preventive maintenance
- Service plan
For further information on our support and services offering please contact your local sales representative.
[Learn More or Contact Us](https://www.sigmaaldrich.com/AT/en/services/product-services/maintenance-and-service-plans/microbiology-maintenance-and-service-plans)
## [](https://www.sigmaaldrich.com)Related Products
### EZ-Fluo™ System Hardware
Product No.DescriptionQty./Pk
[EZFKIT001WW\*](https://www.sigmaaldrich.com/AT/en/product/mm//product/mm/ezfkit001wwm)EZ-Fluo™ System standard kit, including
1 reader, 1 camera and installation CD
1
[EZFREAD01WW\*](https://www.sigmaaldrich.com/AT/en/product/mm/ezfread01ww)EZ-Fluo™reader1
[EZFCAM001](https://www.sigmaaldrich.com/AT/en/product/mm/ezfcam001)EZ-Fluo™ camera1
### EZ-Fluo™ Consumables
Product No.DescriptionQty./Pk
[EZFREAG57](https://www.sigmaaldrich.com/AT/en/product/mm/ezfreag57)
EZ-Fluo™Reagent Kit
57 tests
[PD20047S0](https://www.sigmaaldrich.com/AT/en/product/mm/pd20047s0)
Petri-Pad™ Petri dish, sterile, 47 mm
150
[PD20047S5](https://www.sigmaaldrich.com/AT/en/product/mm/pd20047s5)
Petri-Pad™ Petri dish, sterile, 47 mm
600
\*Country code to be defined at ordering step.
__Related Articles__
- [Fluorescence-based Rapid Microbial Detection](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/microbiological-testing/bioburden-testing/milliflex-quantum-rapid-detection)
- [Compliant and Accurate Microbial Testing with the EZ Product Family](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/microbiological-testing/bioburden-testing/compliant-accurate-microbial-testing-ez-product-family)
- [Assessing Level of Bacteria Purified Water](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/microbiological-testing/bioburden-testing/assessing-level-of-bacteria-purified-water)
__Related Product Categories__
- [Microbial Culture Media](https://www.sigmaaldrich.com/AT/en/products/industrial-microbiology/microbial-culture-media)
- [Microbial Filtration](https://www.sigmaaldrich.com/AT/en/products/industrial-microbiology/microbial-filtration)
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