MITOISO3

Yeast Mitochondria Isolation Kit

sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells

• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• Quality Segment: 200
• usage: sufficient for 40 applications (using 20 OD culture preparations)
• storage temp.: −20°C

Description

General description

Mitochondria, the site of most of the energy production in eukaryotic cells, are characterized by a double membrane structure, an outer membrane and folded inner membrane. Isolated mitochondria may be used as tools for in vitro studies such as respiration and energy production studies, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling.

Application

The kit contains all the reagents required for yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation. In addition, the kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies and a storage buffer for use with intact mitochondria. Yeast Mitochondria Isolation Kit has been used in the isolation and extraction of mitochondria.

Features and Benefits

• The kit′s features include speed, sensitivity, ease of use, and specificity
• The kit contains all the reagents required for a fast and easy yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation
• The kit provides two alternatives for cell membrane disruption. The first one by a detergent and second one by homogenization.
• The kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies
• The kit includes a storage buffer for use with intact mitochondria.
• The reagents are sufficient for 40 procedures using 20 OD culture preparations
• The kit was tested on Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombe

Kit Components Also Available Separately

• L2524

  Lyticase from Arthrobacter luteus, lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

  SDS
• P8215

  Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

  SDS
• C0356

  Protein Extraction Reagent Type 4

  SDS

Safety Information

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302,H314,H334,H351,H361fd,H410
• pcodes: P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Corr. 1B
• Storage Class: 8A - Combustible corrosive hazardous materials
• wgk: WGK 3