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Merck
  • The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.

The application of sodium deoxycholate and Sephacryl-200 for the delipidation and separation of high density lipoprotein.

Experientia (1982-04-15)
H Robern
PMID7084403
ABSTRACT

A method to remove lipids from human plasma high-density lipoprotein (HDL) was developed. The procedure required column chromatography on Sephacryl-200 in the presence of the bile salt sodium deoxycholate. The lipid free protein obtained retained the immunological properties of the native HDL.

MATERIALS
Product Number
Brand
Product Description
S200HR
Sigma-Aldrich
Sephacryl®, 200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)
S400HR
Sigma-Aldrich
Sephacryl®, 400-HR, MW range 20-8000 kDa (globular proteins)
S100HR
Sigma-Aldrich
Sephacryl®, 100-HR, MW range 1000-100,000 Da (globular proteins)
S300HR
Sigma-Aldrich
Sephacryl®, 300-HR, MW range 10-1500 kDa (globular proteins), MW range 1-400 kDa (dextrans)
S500HR
Sigma-Aldrich
Sephacryl®, 500-HR, MW range 40-20,000 kDa (dextrans)