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Virus-like Particle (VLP) Vaccine Bioprocessing and Formulation Steps

This technical article breaks down the steps of upstream and downstream bioprocessing and formulation of virus-like particle vaccines.

Cell and Gene Therapy Manufacturing: Upstream Processing for AAV and Lentivirus Platforms

Minimize the complexities of cell and gene therapy production. Switch to upstream viral vector platforms to enhance scalability and simplify GMP manufacturing.

Bioreactor Process Development and Scale-Up for Transient Transfection-Based Lentivirus Manufacturing in Suspension Cell Culture

A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.

Scalability Challenges of Viral Vector Manufacturing

Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.

Bioprocessing And Scale-Up Of Suspension HEK293 Cells For AAV Manufacturing

To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.

Development of a Novel Sf9 Rhabdovirus-Negative Cell Line (Sf-RVN®) and Companion Chemically Defined Medium

See how the Sf9 rhabdovirus-free cell line was developed and how we’ve developed a companion chemically-defined insect cell media for protein and viral expression.

Quality Materials for Viral Vector Production Security

Raw material selection's critical role in viral vector production for gene therapy includes regulatory guidelines and supplier collaboration importance.

Workflow for the Analysis of Polysorbate 80 in Erbitux® Formulation

A complete reversed phase HPLC-ELSD workflow, including sample preparation for the analysis of Polysorbate 80 in a monoclonal antibody formulation.

An Introduction to Antibodies: Antibody-Antigen Interaction

The strength of interaction between antibody and antigen at single antigenic sites can be described by the affinity of the antibody for the antigen.

Antibody Standard Validation

Explore antibody validation, including antibody and target, species validation, lot validation, application validation, storage and handling, citations, and quality control, in detail.

An Introduction to Antibodies: Antigens, Epitopes and Antibodies

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

Protein Conformation Array Multiplexing

Monoclonal antibodies (mAbs) lead biologics growth, predicted $125 billion worldwide sales by 2020, emphasizing their therapeutic significance.

Conferma^® ELISAs: Designed for Sample Detection & Lot Consistency

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

ELISA Troubleshooting Guide

Troubleshoot and optimize ELISAs using this guide that includes solutions to some of the most common sources of problems for assay development.

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Antibody Generation | Monoclonal vs Polyclonal Antibodies

Learn differences in monoclonal vs polyclonal antibodies including how antibodies are generated, clone numbers, and antibody formats.

A Highly Sensitive IFN-γ ELISpot Assay to Quantify Cellular Immune Responses to Previous Viral Infection

This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).

Secondary Antibodies, Conjugates, and Kits

Technical Support for Secondary Antibodies, Conjugates, and Kits

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Enzymatic Method for Determining Branched Chain Amino Acids (BCAA Assay)

Assay protocol for the colorimetric detection of Branched Chain Amino Acids (BCAA) in biological samples using the alanine assay kit.

Heterocycle and Cyclic Peptide Formation with N-(isocyamino)triphenylphosphorane (Pinc)

Isocyanides are widely used reagents in organic synthesis, with applications ranging from materials science to drug discovery.

Fmoc SPPS Linkers

Novabiochem® offers a wide range of linkers and derivatized resins for Fmoc solid-phase peptide synthesis with specialized protocols.

Solving Aspartimide Formation in Fmoc SPPS with Fmoc-Asp(OBno)-OH

Aspartimide formation 1,2 is caused by repeated exposure of aspartic acid-containing sequences to bases like piperidine and can result ultimately in the generation of 9 different by-products.

Peptide Coupling Reagents Guide

Novabiochem® coupling reagents for in situ activation are fast-reacting and compatible with various amino acids.

Proline Derivatives and Analogs

Proline analogues are promising candidates for tuning the biological, pharmaceutical, or physicochemical properties of naturally occuring, as well as de novo designed, linear, and, cyclic peptides.

Selecting Orthogonal Building Blocks

Novabiochem® offers orthogonally protected amino acids for peptide synthesis, including cyclic and branched peptides.

Trifunctional Probe Building Blocks

In partnership with Pfizer chemists, we have compiled a collection of trifunctional building blocks to enable development of probes.

Unnatural Amino Acids for Peptide Synthesis

Unnatural amino acids, the non-proteinogenic amino acids that either occur naturally or are chemically synthesized, are becoming more and more important as tools for modern drug discovery research.

Micro Photochemical Reactor

Photocatalysis in organic synthesis has grown with innovative methods using visible light, contributing to new research.

Nicewicz Photoredox Catalysts for Anti-Markovnikov Alkene Hydrofunctionalization

Explore Nicewicz Photoredox catalysts—photoexcitable acridinium salts for efficient, metal-free anti-Markovnikov hydrofunctionalizations.

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