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Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography.

Maintenance of Multimodal Chromatography Media and Storage Conditions

This page describes the maintenance of media and storage conditions for multimodal chromatography using Cytiva products.

Characterization of Stable Isotope Caffeine as Reference Material for Isotope Dilution Mass Spectrometry

This technique is based on the direct proportionality of mass fraction ratio and signal intensity ratio of the natural isotope and the C-13 labeled caffeine.

Cannabinoid CRMs: Testing Accuracy & Traceability

ISO-certified cannabinoid CRMs, THC and THCA, available as single components or mixes, for accurate potency testing.

Isotopically Labeled Steroid Standards

Anabolic steroid testing remains crucial in competitive sports to maintain fairness.

The Proper Storage and Handling of Volatile Analytical Standards

Properly storing and handling standards is critical to achieving accurate and reproducible analytical results.

Synthetic Cannabinoid Analysis

A large variety of cannabinoid drug standards, along with an HPLC method with the Supelco Ascentis® Express RP-Amide column, are presented in this article.

Certified Reference Materials for ¹⁹F NMR

Introduction of 19F qNMR certified reference materials for complex molecule certification.

Factors Affecting Analyte Binding on Membrane Filters: A Guide for Filter Validation in Pharmaceutical Analytical QC

Syringe membrane filter selection and validation methods to assess analyte loss due to membrane filter adsorption in pharmaceutical quality control (QC) testing.

Integrity Test Kit User Guide

Integrity Test Kit verifies small volume filter units' integrity, enhancing filtration reliability in lab processes.

Universal Hydrophilic Syringe Filters: Millex^®

Millex® hydrophilic PTFE syringe filters offer broad chemical compatibility and robust performance for various sample filtrations.

Biodiesel

Learn more about Biodiesel as a renewable, alternative diesel fuel produced from vegetable oils, animal fats, or recycled restaurant grease.

Bioethanol

Learn more about Bioethanol as a renewable, alternative fuel traditionally produced by the yeast fermentation of sugar.

Choosing a Capillary GC Column

Optimize gas chromatography separations with suitable columns, considering stationary phase, internal diameter, film thickness, and column length.

Effect of Container Materials on Milk Off-Flavors

HS-SPME with Carboxen®/PDMS coated Nitinol fiber allows low MW analyte detection in light-exposed milk plastics.

SupraSolv® GC Solvent Specifications

Our GC solvents have been specially developed and tested for ECD, FID and MS detection or for headspace gas chromatography to ensure accurate, reliable and reproducible analytical results.

GC Analysis of a 37-Component FAME Mix on Omegawax®

This technical article will discuss GC Analysis of a 37-Component FAME Mix on Omegawax.

Principal Component Analysis (PCA) Evaluation of Seven Commercial Ionic Liquid Capillary GC Columns

In order to cover a broad range of possible solute-stationary phase interactions, 95 solutes with varied functional groups were selected to perform stationary phase characterization.

Radiello Sampler: Monitoring 1,3-Butadiene & Isoprene

Microbial test method development: Laboratory develops compliant methods for antimicrobial products, ensuring regulatory compliance and quality.

Resolving by Mass

The Derivatization and Analysis of Amino Acids by GC-MS

Biopolymer Separations

Supelco’s product offering for biopolymer separations includes columns and media categorized by separation mode, as well as by column brand.

Difluoroacetic Acid as an Effective Mobile Phase Modifier for the LC-UV/MS Analysis of Proteins

The article describes the use of difluoroacetic acid (DFA) as an effective alternative to acid modifiers, formic acid (FA) and trifluoroacetic acid (TFA) in the LC-UV/MS analysis of proteins.

HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns

Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan

HPLC Columns for Antibody and Large Protein Analysis

BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.

An LC/MS Peptide Standard for Rapid System Suitability Assessment and Retention Time Translation Across LC/MS Platforms

Standards are critical in liquid chromatography/mass spectrometry (LC/MS) based proteomics to ensure optimal and consistent system performance before, during, and after sample analysis.

Middle-Up Antibody Analysis by RP Chromatography

Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.

Oligonucleotide Standard 6 Mix LC-UV Analysis

Chromolith® RP-18e columns optimize Oligo Standard 6 separation with varied flow rates and ion-pairing reagent evaluation.

Optimising the LC-MS Analysis of Biomolecules

We present an article concerning optimising the LC-MS analysis of biomolecules.

Application Note – Peroxidase in Vegetables

Application Note: Peroxidase in vegetables – Visual determination after conversion of a specific substrate.

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