[Skip to Content](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/AT/en) Products Cart0 ATEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/AT/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/AT/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/AT/en)[Gene Expression & Silencing](https://www.sigmaaldrich.com/AT/en/applications/genomics/gene-expression-and-silencing)Vector Maps # Vector Maps The MISSION research and development team, in collaboration with the [RNAi Consortium](https://portals.broadinstitute.org/gpp/trc1/user/login) (TRC) scientists, has generated a series of vectors to enable the successful implementation of your RNAi experiments. The base vector pLKO.1-puro was developed1 at the Broad Institute as part of TRC. Our contribution to TRC is a diverse set of vectors for critical control experiments, as well as vectors for custom shRNA production. This page contains information pertaining to these vectors. [pLKO.1-puro sequence (without shRNA insert)](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko.txt) [TRC1.5 Vector: pLKO.1-puro](https://www.sigmaaldrich.com#pLKO "TRC1.5 Vector: pLKO.1-puro") [TRC1.5 Control Vectors](https://www.sigmaaldrich.com#controlvectors "Control Vectors") [TRC1.5 Alternate Vector Backbones](https://www.sigmaaldrich.com#alternate_vector "Alternate Vector Backbones") [TRC2 Vector: TRC2-pLKO-puro](https://www.sigmaaldrich.com#TRC2 "TRC2 Vector: TRC2-pLKO-puro") [Inducible shRNA Vectors](https://www.sigmaaldrich.com#inducible "Inducible shRNA Vectors") [Lentiviral Packaging Vectors](https://www.sigmaaldrich.com#lentiviral "Lentiviral Packaging Vectors") [References](https://www.sigmaaldrich.com#References "References") ## [](https://www.sigmaaldrich.com)TRC1.5 Vector: pLKO.1-puro Vector and Use #### TRC1.5 clones are in the exact same vector backbone as the TRC1 clones Features of the pLKO.1-puro vector allow for transient or stable transfection of the shRNA as well as production of lentiviral particles.1 Stable gene silencing is selected using the puromycin selectable marker while self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.2,3 Exclusive to our portfolio, TRC1.5 contains almost 200,000 clones including more than 49,000 validated clones. It combines all of the content from TRC1 plus an additional 39,212 clones targeting 2,661 new human genes and 2,395 mouse genes. Unlike adenovirus or murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific shRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Compared to siRNA and other vector-based systems, pLKO.1-puro provides solutions for long-term knockdown and phenotypic observation, transduction of difficult or sensitive cell lines (non-dividing cells or primary cells), and is an economical renewable resource. With an shRNA insert, the length of the pLKO.1-puro plasmid is 7,086 bp, as indicated in the vector map below. Without an shRNA insert, pLKO.1-puro vector has a length of 7,052 bp. The sequence file provided is the empty pLKO.1-puro vector (7,052 bp). [](https://www.sigmaaldrich.com)__TRC1.5 Vector Map (pLKO.1-puro)__  ## TRC1.5 Vector Description and Features | | | |---------|----------------------------------------------------| | Name | Description | | cppt | Central polypurine tract | | hPGK | Human phosphoglycerate kinase eukaryotic promoter | | puroR | Puromycin resistance gene for mammalian selection | | SIN/LTR | 3' self inactivating long terminal repeat | | f1 ori | f1 origin of replication | | ampR | Ampicillin resistance gene for bacterial selection | | pUC ori | pUC origin of replication | | 5' LTR | 5' long terminal repeat | | Psi | RNA packaging signal | | RRE | Rev response element | ## [](https://www.sigmaaldrich.com)TRC1.5 Control Vectors We offer a variety of controls for conducting the most rigorous and informative shRNA experiments possible. Visit the shRNA controls web page to learn more. | | | | | |----------------------------------------------------------------------------|----------------------------------------|------------------------------------------------------------------------------------------------------------------------------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| | Product | Purpose | Vector Map | Vector File | | [SHC001](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC001 "SHC001") | Empty vector (no shRNA insert) Control | [SHC001](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/204/376/shc001.pdf "SHC001 Vector Map pdf") | [SHC001](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC001.txt "SHC001 Vector File") | | [SHC002](https://www.sigmaaldrich.com "SHC002") | Non-Mammalian shRNA Control | [SHC002](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/355/143/shc002.pdf "SHC002 Vector Map pdf") | [SHC002](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC002.txt) | | [SHC003](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC003 "SHC003") | TurboGFP Control | [SHC003](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/169/011/shc003.pdf "SHC003 Vector Map pdf") | [SHC003](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC003.txt "SHC003 Vector File") | | [SHC004](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC004 "SHC004") | TurboGFP shRNA Control | [SHC004](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/419/352/shc004.pdf "SHC004 Vector Map pdf") | [SHC004](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC004.txt "SHC004 Vector File") | | [SHC005](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC005 "SHC005") | eGFP shRNA Control | [SHC005](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/101/008/shc005.pdf "SHC005 Vector Map pdf") | [SHC005](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC005.txt) | | [SHC007](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC007 "SHC007") | Luciferase shRNA Control | [SHC007](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/160/363/shc007.pdf "SHC007 Vector Map pdf") | [](https://www.sigmaaldrich.com/AT/en/product/sigma/shc007 "SHC007 Vector File")[SHC007](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/SHC007.txt "SHC00 Vector File") | | SHC008 | B2M shRNA Control | SHC008 | SHC008 | | [SHC009](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC009 "SHC009") | ARHGDIA shRNA Control | [SHC009](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/141/592/shc009.pdf "SHC009 Vector Map pdf") | [SHC009](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/246/863/SHC009.txt "SHC009 Vector File") | | SHC010 | CMV-TagCFP™ Control | SHC010 | SHC010 | | [SHC011](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC011 "SHC011") | CMV-TagYFP™ Control | [SHC011](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/167/338/shc011.pdf "SHC011 Vector Map pdf") | [SHC011](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc011-plko1-puro-cmv-tagyfp.txt "SHC011 pLKO.1-puro CMV-TagYFP Vector File") | | [SHC012](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC012 "SHC012") | CMV-TagRFP™ Control | [SHC012](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/370/026/SHC012.pdf "SHC012 Vector Map pdf") | [SHC012](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc012-plko1-puro-cmv-tagrfp.txt "SHC012 pLKO.1-puro CMV-TagRFP Vector File") | | SHC013 | CMV-TagFP635™ Control | SHC013 | SHC013 | | [SHC014](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC014 "SHC014") | UbC-TurboGFP™ Control | [SHC014](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/141/615/SHC014_v2.pdf "SHC014 Vector Map pdf") | [SHC014](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc014-plko1-puro-ubc-turbogfp.txt "SHC014 pLKO.1-puro UbC-TurboGFP Vector File") | | [SHC015](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC015 "SHC015") | UbC-TagFP635™ Control | [SHC015](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/387/shc015.pdf) | [SHC015](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc013-plko1-puro-cmv-tagfp635.txt "SHC015 pLKO.1-puro UbC-TagFP635 Vector File") | | [SHC016](https://www.sigmaaldrich.com/AT/en/product/SIGMA/SHC016) | Non-Target shRNA Control | [SHC016](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/259/151/shc016.pdf) | [SHC016](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc016-plko1-puro-non-target.txt) | ## TRC1.5 Alternate Vector Backbones We offer a variety of alternate vector backbones as part of our standard shRNA offering. With our customizable MISSION shRNA ordering interface, it is possible to order TRC shRNAs in these vector backbones directly online on the clones detail pages for Plasmid DNA or Lentiviral Transduction Particles. For custom hairpins, please visit our custom cloning page for details on ordering. | | | | | |----------------------|---------------------------|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| | __Product__ | __Purpose__ | __Vector Map__ | __Vector File__ | | pLKO.1-CMV-Neo | Alternate vector backbone | [pLKO.1-CMV-Neo](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/155/123/plko1-cmv-neo-shrna.pdf "pLKO.1-CMV-Neo Vector Map pdf") | [pLKO.1-CMV-Neo](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko1-cmv-neo-shrna.txt) | | pLKO.1-Neo | Alternate vector backbone | [pLKO.1-Neo](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/319/429/plko1-neo-shrna.pdf "pLKO.1-hPGK-Neo Vector Map pdf") | [pLKO.1-Neo](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko1-cmv-neo-shrna.txt) | | pLKO.1-CMV-tGFP | Alternate vector backbone | [pLKO.1-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/153/942/plko1-cmv-tgfp.pdf "pLKO.1-CMV-tGFP Vector Map pdf") | [pLKO.1-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/516/964/pLKO1-CMV-tGFP.txt "pLKO.1-CMV-tGFP Vector File") | | pLKO.1-Neo-CMV-tGFP | Alternate vector backbone | [pLKO.1-Neo-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/108/350/plko1-hpgk-neo-cmv-tgfp.pdf "pLKO.1-hPGK-Neo-CMV-tGFP Vector Map pdf") | [pLKO.1-Neo-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko1-neo-cmv-turbogfp-shrna.txt "pLKO.1-Neo-CMV-tGFP Vector File") | | pLKO.1-puro-CMV-tGFP | Alternate vector backbone | [pLKO.1-puro-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/187/275/plko1-puro-cmv-tgfp.pdf "pLKO.1-puro-CMV-tGFP Vector Map pdf") | [pLKO.1-puro-CMV-tGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/Plko1purocmvtgfp.txt "pLKO.1-puro-CMV-tGFP Vector File") | We also offer several alternate vector backbones with fluorescent proteins or the UbC promoter. These constructs are available only as custom options. Please visit our custom cloning page for details on ordering. | | | | | |---------------------------|---------------------------|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| | Product | Purpose | Vector Map | Vector File | | pLKO.1-puro-CMV-TagCFP™ | Alternate vector backbone | [pLKO.1-puro-CMV-TagCFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/308/460/plko1-puro-cmv-tagcfp.pdf) | [pLKO.1-puro-CMV-TagCFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc010-plko1-puro-cmv-tagcfp-shrna.txt "pLKO.1-puro-CMV-TagCFP Vector File") | | pLKO.1-puro-CMV-TagYFP™ | Alternate vector backbone | [pLKO.1-puro-CMV-TagYFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/429/447/plko1-puro-cmv-tagyfp-shrna.pdf "pLKO.1-puro-CMV-TagYFP Vector Map pdf") | [pLKO.1-puro-CMV-TagYFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc012-plko1-puro-cmv-tagrfp-shrna.txt "pLKO.1-puro-CMV-TagYFP Vector File") | | pLKO.1-puro-CMV-TagRFP™ | Alternate vector backbone | [pLKO.1-puro-CMV-TagRFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/661/654/plko1-puro-cmv-tagrfp-shrna.pdf "pLKO.1-puro-CMV-TagRFP Vector Map pdf") | [pLKO.1-puro-CMV-TagRFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc012-plko1-puro-cmv-tagrfp-shrna.txt "pLKO.1-puro-CMV-TagRFP shRNA Vector File") | | pLKO.1-puro-CMV-TagFP635™ | Alternate vector backbone | [pLKO.1-puro-CMV-TagFP635](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/303/468/plko1-puro-cmv-tagfp635-shrna.pdf "pLKO.1-puro-CMV-TagFP635 Vector Map pdf") | [pLKO.1-puro-CMV-TagFP635](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/726/928/shc013-plko1-puro-cmv-tagfp635-shrna.txt "pLKO.1-puro-CMV-TagFP635 shRNA Vector File") | | pLKO.1-puro-UbC-TurboGFP™ | Alternate vector backbone | [pLKO.1-puro-UbC-TurboGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/405/968/plko1-puro-ubc-turbogfp.pdf) | [pLKO.1-puro-UbC-TurboGFP](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc014-plko1-puro-ubc-turbogfp-shrna.txt "pLKO.1-puro-UbC-TurboGFP shRNA Vector File") | | pLKO.1-puro-UbC-TagFP635™ | Alternate vector backbone | [pLKO.1-puro-UbC-TagFP635](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/324/387/shc015.pdf) | pLKO.1-puro-UbC-TagFP635 | ## TRC2 Vector: TRC2-pLKO-puro Vector and Use The TRC2-pLKO-puro vector also allows for transient or stable transfection of the shRNA as well as the production of lentiviral particles. The only change between the TRC1.5 vector and the TRC2 vector is the addition of the WPRE (or the Woodchuck Hepatitis Post-Transcriptional Regulatory Element).4 This allows for enhanced expression of transgenes delivered by lentivirus.5 As with TRC1.5, stable gene silencing is achieved by using the puromycin selectable marker. Self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. TRC2-pLKO-puro provides solutions for stable long-term knockdown and phenotypic observation, transduction of difficult or sensitive cell lines (non-dividing cells or primary cells), and enhanced expression of transgenes. With an shRNA insert, the length of the TRC2-pLKO-puro plasmid is 7,518 bp, as indicated in the vector map below. Without an shRNA insert, the TRC2-pLKO-puro vector has a length of 7,484 bp. The sequence file provided is the empty TRC2-pLKO-puro vector (7,484 bp). TRC2-pLKO-puro sequence (without shRNA insert)  __TRC2 Vector Map (TRC2-pLKO-puro)__  | | | |---------|-------------------------------------------------------------| | Name | Description | | cppt | Central polypurine tract | | hPGK | Human phosphoglycerate kinase eukaryotic promoter | | puroR | Puromycin resistance gene for mammalian selection | | WPRE | Woodchuck Hepatitis Post-Transcriptional Regulatory Element | | SIN/LTR | 3' self inactivating long terminal repeat | | f1 ori | f1 origin of replication | | ampR | Ampicillin resistance gene for bacterial selection | | pUC ori | pUC origin of replication | | 5' LTR | 5' long terminal repeat | | Psi | RNA packaging signal | | RRE | Rev response element | TRC2 Vector Description and Features | | | | | |----------------------------------------------------------------------------|---------------------------------------------|--------------------------------------------------------------------------------------------------------------------------------------------|-------------------------------------------------------------------------------------------------------------------------------------------------------------------| | __Product__ | __Purpose__ | __Vector Map__ | __Vector File__ | | [SHC201](https://www.sigmaaldrich.com/AT/en/product/sigma/SHC201 "SHC201") | Empty TRC2 vector (no shRNA insert) Control | [SHC201](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/363/595/shc201_vector_map.pdf "SHC201 Vector Map pdf") | [SHC201](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc201_plko-puro.txt "SHC201 Vector File") | | [SHC202](https://www.sigmaaldrich.com/AT/en/product/sigma/SHC202 "SHC202") | Non-Target shRNA Control | [SHC202](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/150/531/shc202_vector_map.pdf "SHC202 Vector Map pdf") | [SHC202](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc202_plko-puro_non-target_shrna.txt "SHC202 Vector File") | | [SHC203](https://www.sigmaaldrich.com/AT/en/product/sigma/SHC203 "SHC203") | TurboGFP Control | [SHC203](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/305/613/shc203_vector_map.pdf "SHC203 Vector Map pdf") | [SHC203](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc203_plko-puro_cmv-turbogfp.txt "SHC203 Vector File") | | [SHC204](https://www.sigmaaldrich.com/AT/en/product/sigma/SHC204 "SHC204") | TurboGFP shRNA Control | [SHC204](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/344/799/shc204_vector_map.pdf "SHC204 Vector Map pdf") | [SHC204](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc204_plko-puro_shrna_to_turbogfp.txt "SHC204 Vector File") | TRC2 Control Vectors Please note: At this time, there are no alternate vector backbones available for TRC2. ### [](https://www.sigmaaldrich.com)Inducible shRNA Vectors The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl-β-D-thio-galactoside), an analogue of lactose, LacI binds to LacO preventing expression of the shRNA. When IPTG is present, the allosteric LacI repressor changes conformation, releasing itself from lacO modified human U6 promoter, and subsequently allows expression of the shRNA. We are proud to offer two different IPTG inducible vectors for your research. The preferred inducible vector, [pLKO-puro-IPTG-3xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/147/343/plko_iptg_3xlaco.pdf), contains three lac operon sequences (two in the U6 promoter and one 3' of the promoter) affording both tight regulation and great gene silencing. Whereas, the [pLKO-puro-IPTG-1xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/200/058/plko_iptg_1xlaco.pdf)  vector contains a single lac operon sequence in the U6 promoter, which allows for an advantage to shRNA expression, but looser control of the promoter when not induced. | | | | | |-----------------------|---------------------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------| | __Product__ | __Purpose__ | __Vector Map__ | __Vector File__ | | pLKO-puro-IPTG-1xLacO | Alternate vector backbone | [pLKO-puro-IPTG-1xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/200/058/plko_iptg_1xlaco.pdf "pLKO-puro-IPTG-1xLacO Vector Map pdf") | [pLKO-puro-IPTG-1xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko-iptg-1xlaco.txt "pLKO-puro-IPTG-1xLacO Vector File") | | pLKO-puro-IPTG-3xLacO | Alternate vector backbone | [pLKO-puro-IPTG-3xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/147/343/plko_iptg_3xlaco.pdf "pLKO-puro-IPTG-3xLacO Vector Map pdf") | [pLKO-puro-IPTG-3xLacO](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/plko_iptg_3xlaco.txt "pLKO-puro-IPTG-3xLacO Vector File") | ## [](https://www.sigmaaldrich.com)Lentiviral Packaging Vectors Finally, for those wanting to package their own lentivirus, we offer the optimized packaging mix. Visit the Lentiviral packaging mix web page to learn more. __Product____Purpose____Vector Map____Vector File__ [SHP001](https://www.sigmaaldrich.com/AT/en/product/sigma/shp001 "SHP001") pMISSIONgagpolPackaging Vector #1[pMISSIONgagpol](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/345/603/pmissiongagpol.pdf) [pMISSIONgagpol](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/pMISSIONgagpol.txt) [SHP001](https://www.sigmaaldrich.com/AT/en/product/sigma/shp001 "SHP001") pMISSIONvsvgPackaging Vector #2[pMISSIONvsvg](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/307/260/pmissionvsvg.pdf "pMISSIONvsvg Vector Map pdf") [pMISSIONvsvg](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/pcmv-vsv-g.txt) ### References 1\. Stewart, S. A. et al. Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA 2003, 9, 493–501.. [https://doi.org/10.1261/rna.2192803](https://doi.org/10.1261/rna.2192803) 2\. Zufferey, R. et al. Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat. Biotechnol 1997, 15, 871–85.. [https://doi.org/10.1038/nbt0997-871](https://doi.org/10.1038/nbt0997-871) 3\. Zufferey, R. et al. Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J. Virol. 1998, 72, 9873–80.. [https://doi.org/10.1128/JVI.72.12.9873-9880.1998](https://doi.org/10.1128/JVI.72.12.9873-9880.1998) 4\. Donello J. E. et al. Woodchuck hepatitis virus contains a tripartite posttranscriptional regulatory element. J. Virol. 1998, 72, 5085-92.. [https://doi.org/10.1128/JVI.72.6.5085-5092.1998](https://doi.org/10.1128/JVI.72.6.5085-5092.1998) 5\. Zufferey, R. et al. Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J. Virol. 1999, 73, 2886-92.. [https://doi.org/10.1128/JVI.73.4.2886-2892.1999](https://doi.org/10.1128/JVI.73.4.2886-2892.1999) ### Contact Us For questions about the library, pricing and quotes or other concerns, please e-mail us at: [[email protected]](mailto:[email protected]).  __Related Articles__ - [pLKO-1 Vector and shRNA Design Frequently Asked Questions](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/genomics/gene-expression-and-silencing/plko-1-vector-shrna) - [Bioluminescent Firefly Luciferase Assays](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/firefly-luciferase-assays) - [Introduction to Cell Transfection](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/transfection-reagents) - [X-tremeGENE™ siRNA Transfection Reagent](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtg-cotransfect-dna-sirna) - [Reverse Transfection of Plasmid DNA](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtg-reverse-transfect-pdna) - [Safe Lentivirus Handling](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/genomics/advanced-gene-editing/safe-handling-of-lentivirus) - [Successful Transduction Using Lentivirus](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/genomics/advanced-gene-editing/successful-transduction-lentivirus) - [esiRNA FAQs (Frequently Asked Questions)](https://www.sigmaaldrich.com/AT/en/technical-documents/technical-article/genomics/functional-genomics-screening/esirna-faq) - [View More](https://www.sigmaaldrich.com/AT/en/search/facet-search?focus=sitecontent&term=facet-search) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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