164598

Aminopeptidase M, Porcine Kidney

• Synonym(s): Aminopeptidase M, Porcine Kidney, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N
• CAS Number: 9054-63-1
• EC Number: 3.4.11.2 (BRENDA, IUBMB)
• MDL number: MFCD00131501
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: Porcine kidney
• Quality Level: 100
• form: suspension (crystalline)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: physiological buffer: soluble; water: soluble
• foreign activity: Oxidases ≤3%
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Aminopeptidase M (APM) is abundantly found in brush border membranes of the kidney, small intestine, placenta, and liver. It is a type II integral membrane protein, known as ectoenzyme.[1]

Application

Aminopeptidase M, porcine kidney has been used:

• to test and identify the peaks containing d-residue in the allatotropin-related peptide (ATRP)[2]
• to study its oxidative inactivation of enzyme activity, and to screen MIRA ingredients[3]
• for the proteolysis of isolevuglandins (IsoLGs) proteins[4]

Biochem/physiol Actions

Aminopeptidase M (APM) is implicated in terminating the actions of certain neuropeptides, like the enkephalins and angiotensin III in the brain. It also metabolizes specific vasoactive peptides. It functions as a receptor for certain viruses, such as coronavirus 229E.[1] APM is a nonspecific peptidase, which rapidly breaks down l-residue peptides.[2]

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.

Physical form

Crystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.

Other Notes

Wachsmuth, E.E., et al. 1966. Biochemistry5, 169.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable