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HCYTA-60K

MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A

Configurable Human Cytokine/Chemokine/Growth Factor 48-Plex Panel A

Synonym(s):

Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel

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About This Item

NACRES:
NA.84
UNSPSC Code:
12161503
To order a Milliplex® kit, please search for your analyte of interest.
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Quality Segment

product line

Milliplex®

species reactivity

human

packaging

pkg of 1 ea

technique(s)

multiplexing: suitable

input

plasma
serum
cell culture supernatant(s)

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

General description

Cytokines, chemokines, and growth factors are key mediatorsof immune system functions capable of signaling through autocrine, paracrine,and endocrine mechanisms. Their pleiotropic immunomodulatory propertiesallow these biomolecules to react to diverse stimuli and regulate the immuneresponse, either promoting or inhibiting inflammation.

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 48 analytes in human serum, plasma, and cell culture supernatants.

Analytes included: sCD40L, EGF, Eotaxin/CCL11, FGF-2/FGF-basic, FLT-3L, Fractalkine/CX3CL1, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12(p70), IL-13, IL-15, IL-17A/CTLA8, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10/CXCL10, MCP-1/CCL2, MCP-3/CCL7, M-CSF, MDC, MIG/CXCL9, MIP-1α/CCL3, MIP-1β/CCL4, PDGF-AA, PDGF-AB/BB, RANTES/CCL5, TGFα, TNFα, TNFβ/LTA, VEGF-A

Note: RANTES cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

  • 48-Plex Power, Simplified: One kit unlocks 48 biomarkers—replaces multiple ELISAs and slashes costs.
  • More Than 20-Year Multiplexing Legacy: The original multiplex innovator—trusted by top pharma companies for critical assays.
  • Cancer, Autoimmunity, & Beyond: Power research in immunotherapy, sepsis, Alzheimer’s, and Long COVID biomarker discovery.
  • Intuitive Workflow: User-friendly protocols that require minimal training, allowing researchers to achieve publication-ready results without extensive background in multiplex assays.
  • Configurable Assay Design: Configure your panel by selecting specific analytes based on your research needs, allowing for a tailored approach that maximizes relevance and impact.
  • Robust Sample Compatibility: Verified for serum, plasma, PBMC supernatants, and cell/tissue lysates. Tested across normal and disease-state samples for broad applicability.
  • Peer-Reviewed Credibility: Frequently cited in immunology and clinical research publications. Trusted by academic institutions, CROs, and pharma companies.
  • Performance Highlights from Comparative Studies: MILLIPLEX® assays showed highest reproducibility among Luminex®-based kits in a head-to-head study and in disease profiling (e.g., ovarian cancer, sepsis), and MILLIPLEX® assays detected more significantly elevated analytes than competitors.
  • Global Support & Documentation: Backed by extensive technical documentation, protocols, and customer support.

Legal Information

Luminex is a registered trademark of Luminex Corporation
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

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Signal Word

Danger

Target Organs

Respiratory Tract

Storage Class

10 - Combustible liquids

WGK

WGK 2

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - STOT RE 2

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Questions

1–9 of 9 Questions  

  1. Can the Milliplex Human Cytokine/Growth Factor Panel A (HCYTA-60K) measure IFNa2a or IFNA2B?

    1 answer

    1. Based on the antibody and standard specification sheets, the kit detects both IFNa2a and IFNa2B. The capture antibody is listed as an IFNa2B antibody and does not mention IFNa2a. However, the standard is recombinant IFNa2a.

      Helpful?

  2. Could you provide a recommendation for sample preparation for placenta and HCYTA-60K assay?

    1 answer

    1. The Milliplex assays have not been tested or verified for use with placenta tissue homogenates. However, a reference is available using placenta homogenates in the Human Cytokine Panel 1 (HCYTOMAG-60K).

      The samples were prepared as follows:
      Placental homogenate: Fragments of frozen placental tissue were transferred to propylene tubes containing lysis buffer and a cocktail of protease inhibitors. The placenta samples were homogenized using an electric homogenizer on ice. After homogenization, the homogenates were centrifuged, and the protein concentration in the supernatant was measured and stored for further analysis.

      Reference: Molás RB, Ribeiro MR, Ramalho Dos Santos MJC, et al. The involvement of annexin A1 in human placental response to maternal Zika virus infection Antiviral Res. 2020 Jul; 179:104809.

      Helpful?

  3. Could you please provide the expected sample ranges for the Milliplex Human Cytokine Panel A (HCYTA-60K)?

    1 answer

    1. The serum and plasma sample values for the HCYTA-60K kit are provided. It's important to note that these values should be used as a guideline only. This data is based on a small sample size without effort for randomization and is not a true reference range.

      Helpful?

  4. What type of IL-12p40 was detected in the Human Cytokine Panel A (HCYTA-60K) – IL-12p40 monomer or IL-12p40 total (both monomer and homodimer)?

    1 answer

    1. The capture in the assay detects the IL-12p40 monomer, and the standard used is also the IL-12p40 monomer. However, specific testing has not been conducted to determine if it also detects the homodimer.

      Helpful?

  5. When precipitating proteins (albumin and globulins) from plasma or serum samples using an Acetonitrile solution, does cortisol, which is bound to albumin & globulin, dissociate into the solution or get removed along with the protein precipitate?

    1 answer

    1. The treatment with acetonitrile removes the matrix effect, including the proteins, from the samples. This means that the cortisol bound to albumin and other proteins is eliminated. The assay specifically measures the free cortisol (non-bound cortisol).

      Helpful?

  6. Are the standards used in Human Cytokine Panel A expressed in E. Coli?

    1 answer

    1. The IFNa2 protein is expressed in yeast, while IL-12p40, IL-12p70, and IL-27 proteins are expressed in HEK293. All the other proteins are expressed in E. Coli.

      Helpful?

  7. Is there an alternative method if a bath sonicator is not available for sonication of beads as mentioned in the Milliplex protocols? Can vortexing for a longer period serve as an alternative?

    1 answer

    1. The Milliplex assays recommend using a sonicator (water bath, Branson B200 or equivalent). Sonication helps break up any aggregated beads and beads stuck to the vial to reduce aggregation and improve counts. If a bath sonicator is not available, you can vortex for up to 2 minutes.

      Helpful?

  8. Is it better to use EDTA vacutainers than heparinized vacutainers when collecting blood from plasma? Does this apply to both humans and mice?

    1 answer

    1. For our Milliplex assays, EDTA plasma is preferred. Care must be taken when using heparin as an anticoagulant since an excess of heparin will provide falsely high values. Use no more than 10 IU heparin per mL of blood collected.

      Helpful?

  9. Do you have any information about BPA, specifically bisphenol A from the plastic used in the immunoassay kits?

    1 answer

    1. The plastic materials used in the immunoassay kit are BPA-free according to the manufacturer's websites.

      Helpful?

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