Merck
All Photos(3)

F7252

Sigma-Aldrich

Fast Green FCF

Dye content ≥85 %

Synonym(s):
Food green 3
Linear Formula:
C37H34N2O10S3Na2
CAS Number:
Molecular Weight:
808.85
Colour Index Number:
42053
Beilstein:
5718212
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

form

powder

Quality Level

composition

Dye content, ≥85%

mp

290 °C (dec.) (lit.)

solubility

H2O: 1 mg/mL, clear

SMILES string

[Na+].[Na+].CCN(Cc1cccc(c1)S([O-])(=O)=O)c2ccc(cc2)C(=C3/C=CC(\C=C3)=[N+](\CC)Cc4cccc(c4)S([O-])(=O)=O)\c5ccc(O)cc5S([O-])(=O)=O

InChI

1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2

Inchi Key

RZSYLLSAWYUBPE-UHFFFAOYSA-L

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General description

Fast Green FCF is one of the triarylmethane dyes,[1] that is biocompatible.[2] This triphenylmethane color additive is mainly used in coloring food, drugs and cosmetics. [2]

Application

Fast Green FCF has been used for in staining of menisci from rabbit[3] and of osteochondral tissues.[4]
Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R.

Packaging

5, 25, 100 g in glass bottle

Biochem/physiol Actions

Fast Green FCF can be considered as a safe stain to detect tumor.[1] In recent years, this has gained importance as an important drug to treat amyloid-deposit diseases.[2]

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Muta. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

352.4 °F - closed cup

Flash Point(C)

178 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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