Merck
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ZRB1956

Sigma-Aldrich

Anti-Axl Antibody, clone 3D13 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Synonym(s):
Tyrosine-protein kinase receptor UFO;EC:2.7.10.1;AXL oncogene

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3D13, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 98.34 kDa
observed mol wt ~140 kDa

purified by

using Protein A

species reactivity

monkey, mouse, human

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... AXL(558)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3D13 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Tyrosine-protein kinase receptor Axl (UFO). It targets an epitope within 23 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal, cytoplasmic domain of human Tyrosine-protein kinase receptor Axl (UFO).

Application

Quality Control Testing

Evaluated by Western Blotting in COS-7 and NIH3T3 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Axl (UFO) in COS-7 cell lysate and 1:1,000 dilution detected Axl (UFO) in NIH3T3 cell lysate.

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Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Axl in U-251 MG cells.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected Axl (UFO) in one million U251 MG cells.

Affinity Binding Assay: A representative lot of this antibody bound Axl (UFO) peptide with a KD of 3.0 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Axl (UFO) in human skeletal muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Tyrosine-protein kinase receptor UFO (UniProt: P30530; also known as EC:2.7.10.1, AXL oncogene) is encoded by the AXL (also known as UFO) gene (Gene ID: 558) in human. AXL is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-25), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 26-451), a transmembrane domain (aa 452-472), and a cytoplasmic domain (aa 473-894). It serves as receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to its ligand the growth arrest specific protein 6 (GAS6) via amino acids 26-92. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL that activates its kinase activity. Its protein kinase domain is localized in amino acids 536-807. Following its activation, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3. Other known downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and PI3-kinase regulatory subunits by AXL also leads to the downstream activation of the Akt. AXL regulates several physiological processes including cell survival, cell proliferation, migration, and differentiation. The AXL/GAS6 signaling is associated with tumor growth, metastasis, invasion, epithelial-mesenchymal transition, angiogenesis, and drug resistance. Higher expression of AXL is reported in metastatic colon and breast cancer; however, its expression in normal colon tissue is weak. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zhu, C., et al. (2019). Mol. Cancer. 18; Article 153; Weinger, JG., et al (2008). J. Neurochem. 106(1); 134-146; Berclaz, G., et al. (2001). Ann. Oncol. 12(6); 819-824).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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