Merck
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ZRB1974

Sigma-Aldrich

Anti-DNMT3B Antibody, clone 2N12 ZooMAb® Rabbit Monoclonal

newenhanced validation

recombinant, expressed in HEK 293 cells

Synonym(s):
DNA (cytosine-5)-methyltransferase 3B;EC:2.1.1.37;DNA methyltransferase HsaIIIB;DNA MTase HsaIIIB;M.HsaIIIB

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2N12, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 95.75 kDa
observed mol wt ~95 kDa

purified by

using Protein A

species reactivity

human, monkey

packaging

antibody small pack of 25 μL

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

N-terminal

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... DNMT3B(1789)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2N12 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects DNA (cytosine-5)-methyltransferase 3B (DNMT3B). It targets an epitope within 22 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal region of human DNA (cytosine-5)-methyltransferase 3B (DNMT3B).

Application

Quality Control Testing

Evaluated by Western Blotting in Caco-2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DNMT3B in Caco-2 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected DNMT3B in COS-7 and NTERA-2 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected DNMT3B in Human testis tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound DNMT3B peptide with a KD of 3.2 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A representative lot of this antibody bound DNMT3B in K562 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

DNA (cytosine-5)-methyltransferase 3B (UniProt: Q9UBC3; also known as EC:2.1.1.37, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB) is encoded by the DNMT3B gene (Gene ID: 1789) in human. DNMT3B is a ubiquitous protein that is highly expressed in fetal liver, heart, kidney, and placenta. In several other tissues it is expressed at lower levels. It is the major de novo DNA methyltransferase that is expressed and is active during the early stage of embryonic development, including implantation. It plays a key role in the control of sense-antisense gene expression and the splicing of alternative exons. In addition to its role to methylate centromeric, pericentromeric, and subtelomeric repeats, it also acts as the main enzyme methylating intragenic regions of active genes. DNMT3B shares a high degree of homology with DNMTA3 in its catalytic domain but displays distinct and overlapping functions with DNMT3A. DNMT3B is recruited to target sites in a tightly regulated manner that involves the crosstalk between the de novo methyltransferase, chromatin remodeling complexes, histone modifications, and transcription factors. DNMT3B contains a PWWP domain (aa 225-283) that is essential for targeting to pericentric heterochromatin. Mutations in DNMT3B gene are known to cause immunodeficiency-centromeric instability-facial anomalies syndrome 1 (ICF1), a rare disorder characterized by recurrent infections, facial anomalies, and branching of chromosomes. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gao, L., et al (2020). Nat. Commun. 11(1); Article 3355; Gagliardi, M., et al. (2018). Front. Cell Dev. Biol. 6; 140).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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