Flow cytometry analysis is a technique commonly used to examine physical and chemical characteristics of cells and particles. Antibodies are used in flow cytometry to help characterize markers on the cell surface since they allow for the detection of specific antigens. These antibodies can be directly conjugated to a fluorophore (direct detection) or paired with a fluorescently labeled secondary antibody (indirect detection). By adding specific fluorophores to specific antibodies, cells can be sorted and analyzed simultaneously.
For successful flow cytometry assays and reproducible results, antibodies validated for use in flow cytometry and proper controls are essential. We offer high-quality flow cytometry validated primary antibodies, secondary antibodies, and isotype control antibodies to support all your flow cytometry needs.
Use our Antibody Explorer search tool to view and compare antibodies by clonality, application, species reactivity, conjugate, host species, and form.
Setting up a successful flow cytometry experiment involves the consideration of many factors such as instrument and dye suitability, primary conjugated antibody availability, secondary antibody cross-reactivity, and more. The ColorWheel® flow cytometry portfolio of antibodies, dyes, and isotype controls was designed to minimize the complexity of balancing these factors through an oligo-based proprietary technology that lets the user independently pick their antibody and dye without a conjugation kit, bringing simplicity to your flow cytometry workflow without compromising on quality.
Flow Cytometry Guide
Key Steps in Flow Cytometry Protocols
Multicolor Flow Cytometry: Tips and Tools for Building Flow Cytometry Panels
How to Select Flow Cytometry Dyes: Quick Tips for Flow Cytometry Dye Selection
Flow Cytometry Troubleshooting Guide: How to Improve Flow Cytometry Analysis
How to Choose a Secondary Antibody
Unless otherwise stated in the Product(s) specifications, any Antibody product is sold for internal research use only and may not be used for any other purpose, which includes but is not limited to, any commercial, diagnostic, or therapeutic use. Our validation processes pertain only to research uses and do not confirm or assure that our antibodies can be used for any unauthorized uses as set forth herein.