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Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production

Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.

Renewable Electricity Toolkit

The Renewable Electricity Supplier Toolkit helps suppliers learn renewable electricity basics and how to bring projects to life.

Transforming the Biopharma Supply Chain and Analytics Capabilities with eData

An essential step in the progress towards Pharma 4.0 is the conversion of paper-based data to an electronic format, allowing it to be transferred, received, and available at the right time, in the right place, by those who need it.

Tangential Flow Filtration in the Antibody Drug Conjugate (ADC) Manufacturing Process

Find best practices for using Pellicon® Capsules in ADC processing as well as data from two studies using Pellicon® Capsules and Pellicon® cassettes in the ADC manufacturing process.

Viral Safety in Bioprocessing

Viral safety in bioprocessing relies on a framework of (1) preventing contamination, (2) detecting contamination, and (3) removing or inactivating viral contaminants.

Single-Pass Tangential Flow Filtration Implementation and Scaling

Get practical guidance on using single-pass tangential flow filtration in your downstream process and scaling between capsules and cassettes.

Viscosity Reduction in High Concentration TFF

This overview describes how use of viscosity-reducing excipients can address viscosity challenges in high concentration tangential flow filtration (TFF) and improve process economics.

Bioburden Control in Bioprocessing

Bioburden control in bioprocessing requires assessing the risks, mitigating the risks, and monitoring bioburden levels.

Sterilizing Grade Filtration Unit Operations for Plasmid DNA Processes

We discuss the final filtration step of plasmid DNA manufacturing and its key considerations and challenges. Download our free pDNA downstream purification eBook or ask our experts.

Purification of Extracellular Vesicles with Eshmuno^® Q Resin and Natrix^® Q Membrane

Separation and purification of subpopulations of extracellular vesicles (EVs) using Eshmuno® Q anion exchange resin and Natrix® Q membrane chromatography.

Ammonium in Wastewater

Step-by-step accurate reflectometric determination of ammonium in wastewater with Nessler’s reagent or Indophenol blue using Reflectroquant®system and test strips.

Analytical Method: Analytical Quality Assurance, Standard for COD/Chloride

Preparation of a standard solution for COD/chloride

Cell Density measurement by OD600 Method

Optical density measurement of bacterial cells at 600 nm using pre-programmed method in Spectroquant® Prove spectrophotometers.

K268 Olive Oil Commission Regulation (EEC) No 2568/91 Annex IX

Learn about the UV-spectrometric determination of the K268 nm value of olive oil, assessing the UV absorbance at 268 nm, to ensure quality and compliance with regulatory standards.

K270 Olive Oil Commission Regulation (EEC) No 2568/91 Annex IX

Discover the K270 method for analyzing olive oil quality as per EEC Regulation 2568/91. Access detailed protocols and ensure compliance with standards.

Nitrate Levels in Vegetables

Reflectometric nitrate determination in vegetables using Griess reagent and Reflectoquant® system.

Nitrate Detection in Wastewater

Reflectometric determination of nitrate in wastewater using Griess reagent and Reflectoquant® test strips.

pH of Milk and Milk Products

Learn how to accurately measure the pH of milk and dairy products using MQuant® pH test strips and the StripScan app for quality control.

HPLC Separation: Ginsenosides from American Ginseng

Optimize HPLC method for ginsenoside separation using a mixture, applying it to American Ginseng root, with conditions and chromatograms shown.

Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC

Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.

PCR/qPCR/dPCR Assay Design

PCR workflow's variability influenced by sample source, reverse transcription, and assay design, impacting reproducibility.

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Improving Reproducibility: Best Practices for Antibodies

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.

Ultrafiltration/Diafiltration (UF/DF) in Plasmid DNA Manufacturing

How are ultrafiltration and diafiltration used in plasmid DNA manufacturing and how should you optimize this process? Learn more in our technical article and download our free eBook.

Tangential Flow Filtration in Downstream Bioprocessing

There are many options for tangential flow filtration (TFF) in downstream bioprocessing from multi-use to single-use TFF or batch TFF and single-pass TFF.

Developing and Manufacturing Attenuated Viral Vaccines

Learn more one the attenuated viral vaccines manufacturing process: cell culture, clarification, nuclease treatment, chromatography, and sterile filtration.

Cell Counting Using a Hemocytometer

Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.

Quantification and Quality Control via CCM Fingerprinting

Learn about rapid and cost-effective identification of more than 100 media formulation components using LC-MS cell culture media fingerprinting.

Membrane Prefilters Improve Sterilizing Filter Capacity and Process Economics in Bioprocessing

Prefilters can be used throughout bioprocessing to remove to remove particulates, improve quality and protect sterilizing filters and downstream unit operations.

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