F2442

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

• Synonym(s): Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum
• MDL number: MFCD00132239
• UNSPSC Code: 12352207
• eCl@ss: 32160409
• NACRES: NA.71
• biological source: bovine fetus
• origin: USA origin
• sterility: sterile-filtered
• shipped in: dry ice

Properties

• biological source: bovine fetus
• sterility: sterile-filtered
• product line: FBS Classic
• composition: Bovine IgG, ≤1 mg/mL ; Hemoglobin, ≤20 mg/dL
• origin: USA origin
• technique(s): cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• impurities: ≤10 EU/mL endotoxin
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.[1][2] FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system.[3] FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture.[4][5] It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM),[6] Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM),[7] M199[8] and Roswell Park Memorial Institute medium, RPMI-1640.[6]

Application

Fetal Bovine Serum has also been used as a component of:

• perivascular-resident macrophage-like melanocytes (PVM/M) growth medium[9]
• synthetic oviduct fluid (SOF)[10]
• Eagle′s minimum essential medium (EMEM)[11]
• neurobasal medium[12]
• Hyclone McCoys 5 A medium[13]
• Hanks medium[14]

Biochem/physiol Actions

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Analysis Note

• Endotoxin and hemoglobin tested

• Tested for the presence of bacteria, virus, and mycoplasma
• Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

Tested for the presence of bacteria, virus, mycoplasma, endotoxin and for hemoglobin content. Triple filtered with 0.1 micron membrane under aseptic conditions.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves