Skip to Content
Merck

F5766

Sigma-Aldrich

Monoclonal Anti-Folic Acid antibody produced in mouse

clone VP-52, ascites fluid

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

0.2 ML
€537.00
0.5 ML
€1,070.00

€537.00

Please contact Customer Service for Availability
Request a Bulk Order

About This Item

MDL number:
MFCD00162295
UNSPSC Code:
12352203
NACRES:
NA.46

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

VP-52, monoclonal

contains

15 mM sodium azide

technique(s)

indirect ELISA: 1:500

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
F4639M7398B0287
Monoclonal Anti-Folic Acid antibody produced in mouse clone VP-52, ascites fluid

Sigma-Aldrich

F5766

Monoclonal Anti-Folic Acid antibody produced in mouse

Monoclonal Anti-Fibrinogen antibody produced in mouse clone FG-21, ascites fluid

Sigma-Aldrich

F4639

Monoclonal Anti-Fibrinogen antibody produced in mouse

Monoclonal Anti-β2-Microglobulin antibody produced in mouse clone BM-63, ascites fluid

Sigma-Aldrich

M7398

Monoclonal Anti-β2-Microglobulin antibody produced in mouse

Monoclonal Anti-FITC−Biotin antibody produced in mouse clone FL-D6, purified immunoglobulin, buffered aqueous solution

Sigma-Aldrich

B0287

Monoclonal Anti-FITC−Biotin antibody produced in mouse

clone

VP-52, monoclonal

clone

FG-21, monoclonal

clone

BM-63, monoclonal

clone

FL-D6, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

biotin conjugate

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

isotype

IgG2b

isotype

IgG2a

isotype

IgG1

isotype

IgG1

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Monoclonal Anti-Folic Acid (mouse IgG2b isotype) is derived from the hybridoma produced from the fusion of mouse myeloma cells and splenocytes of an immunized mouse. The normal range for folate in serum is 3-13 ng/ml.

Immunogen

5-Methyltetrahydrofolic acid (5MTHFA) conjugated to KLH

Application

Monoclonal Anti-Folic Acid antibody is suitable for use in western blot (diluted 1:1000).

Biochem/physiol Actions

Folic acid (folate) is a vitamin that regulates one-carbon transfer during the conversion of homocysteine to methionine. Methionine is a precursor of S-adenosylmethionine, which is the main methyl donor for methylation reactions. Thus, folic acid is essential for regulation of DNA methylations and for epigenetic modifications that modulate gene expression. Folate deficiency has been associated with a wide range of disorders including colorectal cancers, depression, neural tube defects and cerebral dysfunctions
Folic acid is essential for normal growth of mammalian cells. Folic acid deficiencies are common in pregnant women, alcoholics, those whose diets do not include raw fruits and vegetables, and people with structural or functional damage to the small intestine. Vitamin B12 and folic acid are metabolically interrelated.
Monoclonal Anti-Folic Acid antibody binds specifically to folate and detects an epitope present on both folic acid and its biologically active analog 5-methyltetrahydrofolic acid (5MTHFA). The product reacts with free folate, folate conjugated to a carrier such as KLH or BSA, and folic acid in human plasma or serum bound to the endogenous Folate Binder. The antibody does not cross-react with tetrahydrofolic acid, folinic acid or dihydrofolic acid.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000120826
0000120827
0000112963
0000108346
0000108347

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Linglei Jiang et al.
Biochimica et biophysica acta. General subjects, 1865(4), 129763-129763 (2020-10-17)
Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest
Clinical utility of serum folate measurement in tertiary care patients: Argument for revising reference range for serum folate from 3.0 ng/mL to 13.0 ng/mL
Singh G, et al.
practical laboratory medicine, 1, 35-35 (2015)
Periconceptional folate deficiency and implications in neural tube defects.
Safi, J., et al.
Journal of Pregnancy, doi: 10-doi: 10 (2012)
Giuseppe Destito et al.
Chemistry & biology, 14(10), 1152-1162 (2007-10-27)
Cowpea mosaic virus (CPMV) is a well-characterized nanoparticle that has been used for a variety of nanobiotechnology applications. CPMV interacts with several mammalian cell lines and tissues in vivo. To overcome natural CPMV targeting and redirect CPMV particles to cells
Folic acid: A positive influence on periodontal tissues during health and disease
George JP, et al.
INTERNATIONAL JOURNAL OF ADVANCED MULTIDISCIPLINARY RESEARCH, 2(3), 145-145 (2013)
View All Related Papers

Related Content

Questions

1–7 of 7 Questions  

  1. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What is the name of the antibody clone for Product No. F5766, Monoclonal Anti-Folic Acid antibody?

    1 answer

    1. The clone is VP-52.

      Helpful?

  4. Will Product No. F5766, Monoclonal Anti-Folic Acid antibody, work in Western blotting?

    1 answer

    1. The use of this antibody for western blotting has been reported in one study on Pseudomonas and tumor cells. See: Leamon, C.P., et al., Cytotoxicity of Folate-Pseudomonas Exotoxin Conjugates Toward Tumor Cells. J. Biol. Chem., 268(33), 24847-24854 (1993). The antibody was used at a 1 to 1000 dilution in Tris buffered saline (TBS) for 1 hour at 23°C.

      Helpful?

  5. What immunogen was used to generate Product No. F5766, Monoclonal Anti-Folic Acid antibody?

    1 answer

    1. The immunogen used was 5-Methyltetrahydrofolic acid (sometimes called 5MTH folic acid) conjugated to keyhole limpet hemocyanin (KLH).

      Helpful?

  6. What is the isotype of Product No. F5766, Monoclonal Anti-Folic Acid antibody?

    1 answer

    1. This antibody is mouse IgG2B.

      Helpful?

  7. What is the concentration of Product No. F5766, Monoclonal Anti-Folic Acid antibody?

    1 answer

    1. The concentration of the antibody is lot specific and can be found on the certificate of analysis. (It is typically in the 1-2 mg/mL range).

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service