SAB1300398

Anti-STK29 (center) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Anti-BR serine/threonine-protein kinase 2, Anti-BRSK2, Anti-PEN11B, STK29, Anti-SAD1B, Anti-Serine/threonine-protein kinase 29
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): immunohistochemistry: 1:50-1:100; indirect ELISA: 1:1000; western blot: 1:100-1:500
• NCBI accession no.: NP_003948
• UniProt accession no.: Q8IWQ3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STK29(9024)

Description

General description

BRSK2 expressed in insect cells specifically phosphorylates WEE1A, CDC25C, and CDC25B in an in vitro assay, but a kinase-dead mutant does not. Overexpression of BRSK2 in HeLa cells results in increased phosphorylation of CDC25C. DNA damage induced by ultraviolet (UV) irradiation or methyl methane sulfonate, but not by ionizing radiation, enhances endogenous BRSK2 kinase activity in a caffeine-sensitive manner and causes translocation of BRSK2 from the cytoplasm to the nucleus. Overexpression of BRSK2 induces G2/M arrest in HeLa cells. Small interfering RNA against BRSK2 partly abrogates UV-induced G2/M arrest. BRSK2 may act as a checkpoint kinase upon DNA damage induced by UV irradiation or methyl methane sulfonate.

Immunogen

STK29 (AAP97726, 265-300)
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human STK29.

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable