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Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Protein Determination by the Bicinchoninic Acid (BCA) Method
A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.
Production of Dual-labelled Proteins
Refinement of protein dual-labeling protocol using EnPresso® B Defined Nitrogen-free showcased by Vernalis, Ltd. for drug discovery.
Creating PLA® Probes
Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic
SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.
Protein Determination using 2-D Quant Kit
2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.
Protein Determination Modified Lowry Method
To standardize a procedure for the determination of protein by modified Lowry.
Duolink® PLA Brightfield Protocol
Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.
Protein Determination by the Warburg-Christian Method
To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.