H8032

Hanahan′s Broth (SOB Medium)

powder microbial growth medium

Properties

• grade: for molecular biology
• Quality Level: 100
• description: recommended for cultivation of recombinant E. coli.
• sterility: non-sterile
• form: powder
• composition: KCl, 0.186 g/L ; MgSO₄, 2.4 g/L ; NaCl, 0.5 g/L ; Tryptone, 20 g/L ; Yeast extract, 5 g/L
• pH: 6.8-7.2(2.8% solution)
• application(s): food and beverages
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Hannahan′s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation. The 0.4% dextrose composition provides the E. coli carbon and energy needed to mend perforations and for replication.

Application

Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.

Packaging

500 g

Features and Benefits

Hannahan′s Broth (SOB) powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Reconstitution

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Other Notes

Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves