287810

2′,7′-Dichlorofluorescin Diacetate

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.

• Synonym(s): 2′,7′-Dichlorofluorescin Diacetate, H₂DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate
• Empirical Formula (Hill Notation): C₂₄H₁₆Cl₂O₇
• CAS Number: 4091-99-0
• Molecular Weight: 487.29
• MDL number: MFCD00128955

Properties

• Quality Level: 100
• Assay: ≥94% (HPLC)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
• InChI key: PXEZTIWVRVSYOK-UHFFFAOYSA-N

Description

General description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and the nitric oxide (NO) in neutrophils and macrophages and for the determination of the overall oxidative stress in toxicological phenomena.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Crow, J.P. 1997. Nitric Oxide 1, 145.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3