Merck
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11697471001

Roche

NBT/BCIP Ready-to-Use Tablets

tablet, pkg of 20 tablets

Synonym(s):
NBT/BCIP

form

tablet

Quality Level

mol wt

Mr 433.6 (BCIP toluidine salt)
Mr 817.7 (NBT)

packaging

pkg of 20 tablets

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

BCIP is the alkaline phosphatase (AP) substrate, which after dephosphorylation is oxidized by NBT to yield a dark-blue indigo precipitating dye. NBT is thereby reduced to a dark-blue precipitating dye and serves to intensify the color reaction making the detection more sensitive.
The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.

Application

NBT/BCIP Ready-to-Use tablets has been used in:
  • in situ hybridization.
  • western blot
  • southern blot
  • northern blot
  • colony and plaque lifts
  • immunohistochemistry
  • immunocytochemistry
Note: NBT/BCIP may be used with both nitrocellulose and nylon membranes.

Preparation Note

Working solution: Preparation of Color Substrate Solution
Add 1 tablet to 10 ml double-dist. water to prepare a ready-to-use staining solution.
Composition of staining solution (pH 9.5) after addition of double-dist. water: 0.4 mg/ml NBT; 0.19 mg/ml BCIP; 100 mM Tris buffer, pH 9.5; 50 mM MgSO4
Information Note: The color of the solution can vary from slightly yellowish-violet to light brown.
Important Note: Prepare the solution shortly before use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

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Certificate of Origin

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SDS

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