C2734

5-Carboxytetramethylrhodamine

• Synonym(s): 5-TAMRA
• Empirical Formula (Hill Notation): C₂₅H₂₂N₂O₅
• CAS Number: 91809-66-4
• Molecular Weight: 430.45
• MDL number: MFCD01073638
• NACRES: NA.47

Properties

• Assay: ≥95% (HPLC)
• Quality Level: 100
• form: solid
• ε (extinction coefficient): ≥85000-97000 at 539-545 nm in methanol
• fluorescence: λ_ex ~543 nm; λ_em ~570 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• InChI: 1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)
• InChI key: YMZMTOFQCVHHFB-UHFFFAOYSA-N

Description

General description

An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

Application

5-Carboxytetramethylrhodamine has been used in fluorophore characterization.

Biochem/physiol Actions

5-Carboxytetramethylrhodamine can be covalently attached to the DNA and is thereby used in studies involving branched and duplex DNA/RNA structures. It is mainly used for single-molecule studies. 5-Carboxytetramethylrhodamine is also used for the labelling of oligosaccharide for laser-induced fluorescence detection.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves