D7008

Dihydroethidium

≥95%

• Synonym(s): 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
• Empirical Formula (Hill Notation): C₂₁H₂₁N₃
• CAS Number: 104821-25-2
• Molecular Weight: 315.41
• Beilstein: 5107482
• MDL number: MFCD00077335
• PubChem Substance ID: 24894069
• NACRES: NA.47

Properties

• Quality Level: 100
• Assay: ≥95%
• form: powder
• solubility: chloroform: 20 mg/mL
• fluorescence: λ_ex 370 nm; λ_em 420 nm (for cytoplasm of living cells); λ_ex 535 nm; λ_em 610 nm (for chromatin of living cells)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
• InChI: 1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
• InChI key: XYJODUBPWNZLML-UHFFFAOYSA-N

Description

Application

Redox indicator. Blue fluorescence until oxidized to ethidium.

Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).

Packaging

Packaged under Argon.

Linkage

B-ring reduced analog of ethidium

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves