F0888

Fura 2-AM

Name: Fura 2-AM
Brand: SIAL

≥95% purity (HPLC), powder

Synonym(s):

Fura-2 pentakis(acetoxymethyl) ester

Slide 1 of 1

Sign Into View Organizational & Contract Pricing

Select a Size

Pricing and availability is not currently available.

About This Item

Empirical Formula (Hill Notation):

C₄₄H₄₇N₃O₂₄

CAS Number:

108964-32-5

Molecular Weight:

1001.85

Beilstein:

8183748

MDL number:

MFCD00036976

UNSPSC Code:

12171500

PubChem Substance ID:

24894734

NACRES:

NA.47

Key Documents

View All Documentation

Product Name

Fura 2-AM, ≥95% (HPLC)

Quality Level

100

Assay

≥95% (HPLC)

form

powder

color

yellow to yellow-green

solubility

acetone: 10 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

−20°C

SMILES string

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O

InChI

1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3

InChI key

VPSRLGDRGCKUTK-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Clinical & Diagnostics

Hematology Stains

Histology Stains

Tissue Diagnostics

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	47989	344905	F0763
Sigma-Aldrich F0888 Fura 2-AM	Sigma-Aldrich 47989 Fura 2-AM	Sigma-Aldrich 344905 FURA 2/AM	Sigma-Aldrich F0763 Fura 2 pentapotassium salt
color yellow to yellow-green	color -	color yellow	color yellow
application(s) diagnostic assay manufacturing hematology histology	application(s) -	application(s) -	application(s) diagnostic assay manufacturing hematology histology
form powder	form solid	form solid	form powder
solubility acetone: 10 mg/mL	solubility DMSO: soluble	solubility -	solubility 1 M NaOH: 10 mg/mL
assay ≥95% (HPLC)	assay ≥95.0% (HPLC)	assay ≥95% (HPLC)	assay ≥80%
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

General description

Fura 2-AM (Fura 2) is a calcium-specific ratio-metric fluorescent dye. It is also known as acetoxy-methyl-ester. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505nm, and the excitation peak ranges between 340-380nm and is the preferred calcium indicator.

Application

Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells.

Features and Benefits

The major benefits of using the ester form of Fura 2 as Fura 2-AM are: It is cell-permeable. It can be loaded into cells easily. Another advantage of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of : The dye concentration. Illumination intensity, and Optical path length.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Effects of Taurine or Trehalose supplementation on functional competence of cryopreserved Karan Fries semen.

Chhillar S

Animal Reproduction, 135, 1-1 (2012)

Role of chondroitin 4-sulphate as a receptor for polycation induced human platelet aggregation.

J L Donato et al.

British journal of pharmacology, 119(7), 1447-1453 (1996-12-01)

1. Proteoglycans provide negatively charged sites on the surface of platelets, leukocytes and endothelial cells. Since chondroitin 4-sulphate is the main proteoglycan present on the platelet surface, the role of this molecule in mediating the activation of human platelets by

Assessment of intracellular Ca2+, cAMP and 1,2-diacylglycerol in cryopreserved buffalo (Bubalus bubalis) spermatozoa on supplementation of taurine and trehalose in the extender.

Singh VK

Reproduction in domestic animals = Zuchthygiene, 47, 584-584 (2012)

The human thromboxane A2 receptor alpha isoform (TP alpha) functionally couples to the G proteins Gq and G11 in vivo and is activated by the isoprostane 8-epi prostaglandin F2 alpha.

B T Kinsella et al.

The Journal of pharmacology and experimental therapeutics, 281(2), 957-964 (1997-05-01)

To establish whether the thromboxane A2 (TXA2) receptor (TP) functionally couples to the Gq family of heterotrimeric G proteins in vivo, we have coexpressed the cDNAs coding for the human platelet/placental TP alpha isoform (TP alpha) and the alpha subunits

Assessment of Fura-2 for measurements of cytosolic free calcium.

M W Roe et al.

Cell calcium, 11(2-3), 63-73 (1990-02-01)

Fura-2 has become the most popular fluorescent probe with which to monitor dynamic changes in cytosolic free calcium in intact living cells. In this paper, we describe many of the currently recognized limitations to the use of Fura-2 in living

View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service