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Cellulase from Aspergillus niger

powder, off-white, ~0.8 U/mg

1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

biological source

Aspergillus niger

Quality Level



specific activity

~0.8 U/mg



storage temp.


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General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.


Cellulase from Aspergillus niger has been used:
  • in the preparation of the standardized solution to determine absolute cellulase levels
  • in phosphate-citric acid buffer for activity assay
  • in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed

Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

Unit Definition

1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C


Health hazard

Signal Word


Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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More documents

Quotes and Ordering

P. Toussaint et al.
Entropie, 23 (136), 8-8 (1987)
G. Okada
Methods in Enzymology, 160, 259-259 (1988)
María Dolores Ayala et al.
Animals : an open access journal from MDPI, 10(12) (2020-12-05)
A 90-d feeding trial was conducted in which five groups of gilthead seabream (11.96 g initial body weight) were fed with a microalgae-free diet (control group, C) or four diets containing the microalgae Nannochloropsis gaditana at two inclusion levels (2.5%
Helena Fernandes et al.
Bioresource technology, 281, 277-285 (2019-03-03)
The macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by Aspergillus ibericus and enzymatic hydrolysis (EH) were applied to washed
Thalita Regina Tuleski et al.
Scientific reports, 9(1), 4041-4041 (2019-03-13)
Three species of the β-Proteobacterial genus Herbaspirillum are able to fix nitrogen in endophytic associations with such important agricultural crops as maize, rice, sorghum, sugar-cane and wheat. In addition, Herbaspirillum rubrisubalbicans causes the mottled-stripe disease in susceptible sugar-cane cultivars as

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