Anti-rabbit IgG (whole molecule) (Sigma, R4880) is developed in goat using purified rabbit IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-rabbit IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to rabbit IgG. Goat anti-rabbit is conjugated to Atto 633 NHS (Abs.max. 629 nm; Em.max. 657 nm), (Cat. No. 01464), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.
purified rabbit IgG
Goat anti-rabbit IgG can be used for immunohistochemical applications at a dilution of 1:200.
unconjugated dye ≤5% of total fluorescence
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.